Mouse anti-Human CD230/Prion Monoclonal Antibody | anti-CD230 antibody
Anti-Hu CD230/Prion Purified
Gene Names
PRNP; CJD; GSS; PrP; ASCR; KURU; PRIP; PrPc; CD230; AltPrP; p27-30; PrP27-30; PrP33-35C
Reactivity
Human
Applications
Western Blot
Purity
Purified by protein-A affinity chromatography
Synonyms
CD230/Prion; Monoclonal Antibody; Anti-Hu CD230/Prion Purified; Major prion protein; PrP; PrP27-30; PrP33-35C; ASCR; CD230; PRNP; CJD; KURU; PRIP; PRPC; prion protein; anti-CD230 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2a
Clone Number
EM-21
Specificity
The mouse monoclonal antibody EM-21 recognizes human prion protein (PrP). Diglycosylated form of PrP has ~ 40kDa, monoglycosylated form ~ 30kDa, and nonglycosylated form ~ 19-21kDa.
Purity/Purification
Purified by protein-A affinity chromatography
Form/Format
Purified. Phosphate buffered saline (PBS) solution with 15mM sodium azide
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD230 antibody
Western Blot (WB)
Application Notes
WB: Recommended dilution: 0.5ug/ml, non-reducing conditions are essential.
Immunogen
Recombinant human prion protein
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Do not use after expiration date stamped on the label.
Related Product Information for anti-CD230 antibody
CD230/Human prion protein (PrP), also known as PRNP, is a ubiquitously expressed GPI-anchored cell surface glycoprotein associating with lipid raft components and Functional Application (FUNC)tioning as a signaling molecule. CD230/PrP plays a role in apoptosis in a cell context-dependent manner, is involved in proliferation of epithelial cells and in distribution of junction-associated proteins in human enterocytes. Conversion of this normal cellular prion protein (PrPc) into an abnormal conformer (PrPSc) is the crucial step associated with triggering the pathogenesis of the prion neurodegenerative disorders, such as the Creutzfeld-Jakob disease (CJD). Whereas PrPc is rich in alpha-helices, the PrPSc form has higher content of beta-sheets and is resistant to proteinase K.
References
*Li C, Yu S, Nakamura F, Yin S, Xu J, Petrolla AA, Singh N, Tartakoff A, Abbott DW, Xin W, Sy MS: Binding of pro-prion to filamin A disrupts cytoskeleton and correlates with poor prognosis in pancreatic cancer. J Clin Invest. 2009 Aug 17. pii: 39542. doi: 10.1172/JCI39542. [Epub ahead of print], URL: https://www.ncbi.nlm.nih.gov/pubmed/19690385ordin,*Ermonval M, Baudry A, Baychelier F, Pradines E, Pietri M, Oda K, Schneider B, Mouillet-Richard S, Launay JM, Kellermann O: PLoS One. 2009 Aug 4;4(8):e6497., URL: https://www.ncbi.nlm.nih.gov/pubmed/19652718ordin,*Yang Y, Chen L, Pan HZ, Kou Y, Xu CM: Glycosylation modification of human prion protein provokes apoptosis in HeLa cells in vitro. BMB Rep. 2009 Jun 30;42(6):331-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/19558790ordin,*Segarra C, Lehmann S, Coste J: Prion protein expression and processing in human mononuclear cells: the impact of the codon 129 prion gene polymorphism. PLoS One. 2009 Jun 4;4(6):e5796., URL: https://www.ncbi.nlm.nih.gov/pubmed/19495414ordin,*Wang X, Dong CF, Shi Q, Shi S, Wang GR, Lei YJ, Xu K, An R, Chen JM, Jiang HY, Tian C, Gao C, Zhao YJ, Han J, Dong XP: Cytosolic prion protein induces apoptosis in human neuronal cell SH-SY5Y via mitochondrial disruption pathway. BMB Rep. 2009 Jul 31;42(7):444-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/19643043ordin
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33kDa
NCBI Official Full Name
major prion protein preproprotein
NCBI Official Synonym Full Names
prion protein
NCBI Official Symbol
PRNP
NCBI Official Synonym Symbols
CJD; GSS; PrP; ASCR; KURU; PRIP; PrPc; CD230; AltPrP; p27-30; PrP27-30; PrP33-35C
NCBI Protein Information
alternative prion protein; major prion protein; CD230 antigen; prion-related protein
UniProt Protein Name
Major prion protein
UniProt Gene Name
PRNP
UniProt Synonym Gene Names
PRIP; PRP; PrP
UniProt Entry Name
PRIO_HUMAN
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Product Notes
The CD230 prnp (Catalog #AAA1801778) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD230/Prion Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD230/Prion can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Recommended dilution: 0.5ug/ml, non-reducing conditions are essential. Researchers should empirically determine the suitability of the CD230 prnp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD230/Prion, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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