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Mouse anti-Human, Mouse CD229 Monoclonal Antibody

CD229 (Ly-9) (PE)

Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Synonyms
CD229; Monoclonal Antibody; CD229 (Ly-9) (PE); Anti -CD229 (Ly-9) (PE); anti-CD229 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3H1998
Specificity
Recognizes the human CD229 cell surface antigen, a 100-120kD cell surface glycoprotein that is the homologue of mouse Ly-9.CD229 is a member of the immunoglobulin superfamily and may have a function in cell signalling. The antigen is expressed at low levels by mature T and B lymphocytes and at higher levels by single-positive thymocytes. Expression at variable levels is also seen in some cell lines, such as Daudi, U937 and HUT78.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, pH 7.2, 0.1% sodium azide, 1% BSA. Conjugated to R. Phycoerythrin (RPE). Reconstitute with 1ml sterile 40-50% glycerol, ddH2O.
Applicable Applications for anti-CD229 antibody
Flow Cytometry (FC/FACS)
Application Notes
Flow Cytometry: Neat, Optimal working dilutions to be determined by researcher.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul or 100ul whole blood.
Immunogen
Mouse pre-B cell line 300.19 transfected with CD229 cDNA.
Recommended Negative Controls
Mouse IgG1 Negative Control: RPE
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Preparation and Storage
-20 degree C
Product Categories/Family for anti-CD229 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI Official Full Name
cytochrome oxidase subunit I

Uniprot Description

Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.

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Product Notes

The CD229 (Catalog #AAA603295) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD229 (Ly-9) (PE) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD229 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Flow Cytometry: Neat, Optimal working dilutions to be determined by researcher. Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul or 100ul whole blood. Researchers should empirically determine the suitability of the CD229 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD229, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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