Rat anti-Mouse CD226 Monoclonal Antibody | anti-CD226 antibody

Anti-Mouse CD226, FITC, (clone: 15F.10E5), (rat IgMk)

Cat. Num. AAA520070

• Gene Names: CD226; PTA1; DNAM1; DNAM-1; TLiSA1
• Reactivity: Mouse
• Applications: Immunohistochemistry
• Synonyms: CD226; Monoclonal Antibody; Anti-Mouse CD226; FITC; (clone: 15F.10E5); (rat IgMk); FITC Anti-Mouse CD226 (DNAM-1) Monoclonal Antibody; anti-CD226 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgMkappa
• Clone Number: 15F.10E5
• Specificity: CD226 (Mouse CD226 (DNAM-1))
• Form/Format: FITC (FITC conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.)
• Concentration: Antibody Concentration: 0.1 mg/ml (varies by lot)
• Sequence Length: 336

• Applicable Applications for anti-CD226 antibody: Immunohistochemistry (IHC)
• Immunogen: AE7 cells
• Donor: Lewis rat
• Fusion Partner: SP 2/0 myeloma
• Presentation: 100 ug or 300 ug FITC conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml
• Preparation and Storage: Store at 4 degree C. For long term storage, aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles.

Testing Data

Related Product Information for anti-CD226 antibody

CD226 (also called DNAM-1) is constituitively expressed on naive CD8+ T cells, as well as subsets of naïve CD11b+ macrophages and NK cells. It is also found on a lower percentage (~40%) of unactivated CD4+ T cells. CD226 is functional upon T cell activation, and CD226 ligands (CD112 and CD155) are the same in the mouse as for human (Tage-4 is the mouse homologue of CD155). The gene for mouse DNAM-1 was identified on chromosome 18, and the predicted amino acid sequence shows a 53% homology with human DNAM-1. Recent experiments show that this antibody (clone 15F.10E5) only binds to differentiated Th1 cells but not Th2 or Th0 cells. It also suppressed both antigen-specific T cell expansion and an autoimmune disease (EAE) mediated by effector Th1 cells. This antibody is suitable for use in flow cytometry.

NCBI and Uniprot Product Information

• NCBI GI #: 171460997
• NCBI GeneID: 10666
• NCBI Accession #: NP_006557.2
• NCBI GenBank Nucleotide #: NM_006566.2
• UniProt Accession #: Q15762; B2R818
• Molecular Weight: 38,614 Da
• NCBI Official Full Name: CD226 antigen
• NCBI Official Synonym Full Names: CD226 molecule
• NCBI Official Symbol: CD226
• NCBI Official Synonym Symbols: PTA1; DNAM1; DNAM-1; TLiSA1
• NCBI Protein Information: CD226 antigen; adhesion glycoprotein; DNAX accessory molecule 1; DNAX accessory molecule-1; platelet and T cell activation antigen 1; T lineage-specific activation antigen 1 antigen
• UniProt Protein Name: CD226 antigen
• Protein Family: CD226 antigen
• UniProt Gene Name: CD226
• UniProt Synonym Gene Names: DNAM1; DNAM-1
• UniProt Entry Name: CD226_HUMAN

NCBI Description

This gene encodes a glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. The protein mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell. Ref.1

Subunit structure: Interacts with PVR and PVRL2. Ref.7 Ref.8

Subcellular location: Membrane; Single-pass type I membrane protein

Potential.

Tissue specificity: Expressed by peripheral blood T-lymphocytes. Ref.1

Sequence similarities: Contains 2 Ig-like C2-type (immunoglobulin-like) domains.