Nexilin Recombinant Protein | NEXN recombinant protein

Recombinant Nexilin (NEXN)

Cat. Num. AAA2029227

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Nexilin; Recombinant Nexilin (NEXN); NEXN recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; HEDDDVDVRP AKKSESPFTH KVNMKARFEQ MAKAREEEEQ RRIEEQKLLR MQFEQKEIDA ALQKKREDEE EEEGSIVNGS TTEDEEQTRS GAPWFKKPLR NTSVVDSEPV RFTVKVTGEP KPEITWWFEG EILQDGEDYQ YIERGETYCL YLPETFPEDG GEYMCKAVNN KGSAASTCILTIEMDDY
• Sequence Length: 675

• Applicable Applications for NEXN recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 51.7kDa
• Accurate Molecular Mass (KD): 52kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag, GST-tag, S-tag
• Organism Species: Mus musculus (Mouse)
• Fragment: Asp42~Asn293
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 93009033
• Molecular Weight: 72,732 Da
• NCBI Official Full Name: nexilin
• UniProt Protein Name: Nexilin
• Protein Family: Nexilin
• UniProt Gene Name: NEXN
• UniProt Entry Name: NEXN_HUMAN

Uniprot Description

NEXN: Involved in regulating cell migration through association with the actin cytoskeleton. Has an essential role in the maintenance of Z line and sarcomere integrity. Defects in NEXN are the cause of cardiomyopathy dilated type 1CC (CMD1CC). A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Defects in NEXN are the cause of familial hypertrophic cardiomyopathy type 20 (CMH20). CMH20 is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1p31.1

Cellular Component: cytoskeleton; focal adhesion; Z disc

Molecular Function: actin filament binding; structural constituent of muscle; calmodulin-dependent protein kinase activity

Biological Process: regulation of apoptosis; cardiac muscle fiber development; protein amino acid phosphorylation; regulation of cell migration; regulation of cytoskeleton organization and biogenesis

Disease: Cardiomyopathy, Dilated, 1cc; Cardiomyopathy, Familial Hypertrophic, 20