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Testing Data (Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD21)

Mouse CD21 Monoclonal Antibody | anti-CD21 antibody

CD21 (CR2) (MaxLight 490)

Reactivity
Bovine, Goat, Sheep
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Purity
Purified by protein G affinity chromatography from tissue culture supernatant.
Synonyms
CD21; Monoclonal Antibody; CD21 (CR2) (MaxLight 490); anti-CD21 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Bovine, Goat, Sheep
Clonality
Monoclonal
Isotype
IgG1
Clone Number
CC21
Specificity
Recognizes bovine CD21. Species Crossreactivity: Goat, sheep, red deer, mule deer
Purity/Purification
Purified by protein G affinity chromatography from tissue culture supernatant.
Form/Format
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight490.
Applicable Applications for anti-CD21 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Conjugate
MaxLight490
Preparation and Storage
Store at 4 degree C. Do Not Freeze.

Testing Data

(Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD21)

Testing Data (Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD21)
Product Categories/Family for anti-CD21 antibody
References
1. Howard, C.J. et al. (1991) Summary of workshop findings for leukocyte antigens of cattle. Vet. Immunol. Immunopathol. 27: 21-27. 2. Naessens, J. et al. (1990) Characterization of a bovine leucocyte differentiation antigen of 145,000 Mw restricted to B lymphocytes. Immunology 69: 525-530. 3. Sopp, P. & Howard, C.J. (2001) IFN gamma and IL-4 production by CD4, CD8, and WC1 gamma-delta TCR+ cells from cattle lymph nodes and blood. Vet. Immunol. Immunopathol. 81: 85-96. 4. Lwin, S. et al. (2009) Immune cell types involved in early uptake and transport of recombinant mouse prion protein in Peyer's patches of calves. Cell Tissue Res. 338: 343-54. 5. Breugelmans, S. et al. (2011) Immunoassay of lymphocyte subsets in ovine palatine tonsils. Acta Histochem. 113: 416-22. 6. Halliday, S. et al. (2005) Expression of PrPC on cellular components of sheep blood. J. Gen. Virol. 86: 1571-1579. 7. Brackenbury, L.S. et al. (2005) Identification of a cell population that produces alpha/beta interferon in vitro and in vivo in response to noncytopathic bovine viral diarrhea virus. J Virol. 79: 7738-44. 8. Breugelmans, S. et al. (2011) Differences between the ovine tonsils based on an immunohistochemical quantification of the lymphocyte subpopulations. Comp Immunol Microbiol Infect Dis. 34: 217-25. 9. Richt, J.A. et al. (2007) Production of cattle lacking prion protein. Nat Biotechnol. 25: 132-8. 10. Brujeni, G.N. et al. (2010) Bovine immunodeficiency virus and bovine leukemia virus and their mixed infection in Iranian Holstein cattle. J Infect Dev Ctries. 4: 576-9. 11. Kiku, Y. et al. (2010) Decrease in bovine CD14 positive cells in colostrum is associated with the incidence of mastitis after calving. Vet Res Commun. 34: 197-203. 12. Pilla, R. et al. (2012) Long-term study of MRSA ST1, t127 mastitis in a dairy cow. Vet Rec. Mar 1. 13. Chattha, K.S. et al. (2010) Immunohistochemical investigation of cells expressing CD21, membrane IgM, CD32 and a follicular dendritic cell marker in the lymphoid tissues of neonatal calves. Vet Immunol Immunopathol. 137: 284-90. 14. Weiss, D.J. et al. (2006) Mucosal immune response in cattle with subclinical Johne's disease. Vet Pathol. 43: 127-35. 15. Summers, C. et al. (2012) The distribution of immune cells in the lungs of classical and atypical ovine pulmonary adenocarcinoma. Vet Immunol Immunopathol. 146: 1-7. 16. Sigurdson, C.J. et al. (2002) PrP(CWD) lymphoid cell targets in early and advanced chronic wasting disease of mule deer. J Gen Virol. 83: 2617-28. 17. Dagleish, M.P.et al. (2012) Immunophenotype of cells within cervine rectoanal mucosa-associated lymphoid tissue and mesenteric lymph nodes. J Comp Pathol. 146: 365-71. 18. Edwards, J.C. et al. (2010) PrP(Sc) is associated with B cells in the blood of scrapie-infected sheep. Virology. 405: 110-9. 19. Brodzki, P. et al. (2014) Phenotyping of leukocytes and granulocyte and monocyte phagocytic activity in the peripheral blood and uterus of cows with endometritis. Theriogenology 28 April [Epub ahead of print)

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Product Notes

The CD21 (Catalog #AAA6253488) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD21 (CR2) (MaxLight 490) reacts with Bovine, Goat, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's CD21 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the CD21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD21, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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