Mouse CD20 / MS4A1 Monoclonal Antibody | anti-MS4A1 antibody

CD20 / MS4A1 (B-Cell Marker) Mouse Monoclonal Antibody

Cat. Num. AAA438617

• Gene Names: MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
• Reactivity: Human, Baboon, Monkey.; Does not react with Cow, Dog, Pig, and Rat
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Synonyms: CD20 / MS4A1; Monoclonal Antibody; CD20 / MS4A1 (B-Cell Marker) Mouse Monoclonal Antibody; APY; ATOPY; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; Bp35; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; High affinity immunoglobulin epsilon receptor subunit beta; IgE Fc receptor subunit beta; IGEL; IGER; IGHER; LEU16; Leukocyte surface antigen Leu-16; Ly44; Membrane spanning 4 domains subfamily A member 2; Membrane-spanning 4-domains subfamily A member 1 (MS4A1); anti-MS4A1 antibody

• Host: Mouse
• Reactivity: Human, Baboon, Monkey.; Does not react with Cow, Dog, Pig, and Rat
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: SPM494
• Specificity: Recognizes a protein of 30-33kDa, which is identified as CD20. Its epitope is located in the cytoplasmic domain of CD20 and was, therefore, ascribed as CD20cy in the 5th Workshop. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin's disease, particularly of lymphocyte predominant type.
• Form/Format: Tissue culture supernatant with 0.05% Azide.
• Sequence Length: 297

• Applicable Applications for anti-MS4A1 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
• Application Notes: Flow Cytometry (2-5ul/million cells in 0.1ml); Immunofluorescence (1:100-1:200); Immunohistochemistry (Formalin-fixed) (1:100-1:200 for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Predominantly Cell Surface with some cytoplasmic
• Immunogen: Human tonsil B cells
• Hu-Chromosome Location: 11q12.2
• Positive Control: Daudi, Raji, and U266, and human lymphocytes. Lymph nodes and tonsils.
• Preparation and Storage: Store at 2 to 8 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Lymphoma stained with CD20 Monoclonal Antibody (SPM494))

References

Schlossman, S., et al., eds. 1995. Leucocyte Typing V. New York: Oxford University Press.

NCBI and Uniprot Product Information

• NCBI GI #: 23110987
• NCBI GeneID: 931
• NCBI Accession #: NP_068769.2
• NCBI GenBank Nucleotide #: NM_021950.3
• UniProt Accession #: P11836; P08984; Q13963; A6NMS4; B4DT24
• Molecular Weight: 33-37kDa
• NCBI Official Full Name: B-lymphocyte antigen CD20
• NCBI Official Synonym Full Names: membrane spanning 4-domains A1
• NCBI Official Symbol: MS4A1
• NCBI Official Synonym Symbols: B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
• NCBI Protein Information: B-lymphocyte antigen CD20
• UniProt Protein Name: B-lymphocyte antigen CD20
• Protein Family: B-lymphocyte antigen
• UniProt Gene Name: MS4A1
• UniProt Synonym Gene Names: CD20
• UniProt Entry Name: CD20_HUMAN

NCBI Description

This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

MS4A1: This protein may be involved in the regulation of B-cell activation and proliferation. Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5); also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. Belongs to the MS4A family.

Protein type: Cell surface; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: extracellular space

Molecular Function: protein binding

Disease: Immunodeficiency, Common Variable, 5