SDS-Page
(Detection of Bub3. Cell lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1.0ug/ml goat anti-hBub3.)
anti-Human BUB3 Polyclonal Antibody | anti-BUB3 antibody
Anti - BUB3 (BUB3L, Budding uninhibited by benzimidazoles 3, HGNC:1151)
Gene Names
Bub3; bub3; CG7581; dBUB3; Dmbub3; DmelCG7581
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by immunoaffinity chromatography.
Synonyms
BUB3; Polyclonal Antibody; Anti - BUB3 (BUB3L; Budding uninhibited by benzimidazoles 3; HGNC:1151); BUB3 (BUB3L; anti-BUB3 antibody
Host
Host: Goat
Source: Human
Source: Human
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Bub3.
Purity/Purification
Purified by immunoaffinity chromatography.
Form/Format
Affinity Purified
Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
Concentration
~0.2mg/ml (varies by lot)
Sequence Length
326
Applicable Applications for anti-BUB3 antibody
ELISA, Western Blot
Application Notes
Dilution: ELISA: 0.5-1.0ug/ml
Western Blot: 1.0ug/ml
Optimal dilutions to be determined by the researcher.
Western Blot: 1.0ug/ml
Optimal dilutions to be determined by the researcher.
Immunogen
Recombinant human Bub3 aa1-328; O43684 (E. coli).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SDS-Page
(Detection of Bub3. Cell lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1.0ug/ml goat anti-hBub3.)
SDS-Page
(Detection of Bub3. Cell lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1.0ug/ml goat anti-hBub3.)
Related Product Information for anti-BUB3 antibody
Bub3 (Budding uninhibited by benzimidazoles 3 homolog; also Mitotic checkpoint protein Bub3) is a 35-40kD member of the WD repeat Bub3 family of molecules. It plays a key role in chromosome segregation by serving as a component of the mitotic checkpoint complex. This complex inhibits Cdc20, and E3 ubiquitin ligase that initiates the metaphase to anaphase
Product Categories/Family for anti-BUB3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37,415 Da
NCBI Official Full Name
Bub3
NCBI Official Symbol
Bub3
NCBI Official Synonym Symbols
bub3; CG7581; dBUB3; Dmbub3; DmelCG7581
NCBI Protein Information
CG7581 gene product from transcript CG7581-RA; Bub3; CG7581-PA; Bub3-PA
UniProt Protein Name
Bub3
Protein Family
UniProt Gene Name
Bub3
UniProt Synonym Gene Names
Bub3-RA
UniProt Entry Name
Q9VAJ2_DROME
Similar Products
Product Notes
The BUB3 bub3 (Catalog #AAA620639) is an Antibody produced from Host: Goat Source: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti - BUB3 (BUB3L, Budding uninhibited by benzimidazoles 3, HGNC:1151) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BUB3 can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Dilution: ELISA: 0.5-1.0ug/ml Western Blot: 1.0ug/ml Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the BUB3 bub3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BUB3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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