Mouse anti-Human. CD20 Monoclonal Antibody | anti-MS4A1 antibody

CD20

Cat. Num. AAA438098

• Gene Names: MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
• Reactivity: Human.
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
• Synonyms: CD20; Monoclonal Antibody; APY; ATOPY; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; Bp35; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; High affinity immunoglobulin epsilon receptor subunit beta; IgE Fc receptor subunit beta; IGEL; IGER; IGHER; LEU16; Leukocyte surface antigen Leu-16; Ly44; Membrane spanning 4 domains subfamily A member 2; Membrane-spanning 4-domains subfamily A member 1 (MS4A1); anti-MS4A1 antibody

• Host: Mouse
• Reactivity: Human.
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: B9E9
• Specificity: Recognizes a protein of 33-37kDa, identified as CD20 (Workshop V; Code CD20.12). B9E9 recognizes extracellular domain of CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, B cell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in B-cell activation and cell cycle progression.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 297

• Applicable Applications for anti-MS4A1 antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Functional Studies, Immunoprecipitation (IP), Immunohistology (IH)
• Application Notes: ELISA (For coating, order Ab without BSA) Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); Functional Studies (Order Ab without BSA & Azide); Immunoprecipitation (0.5-1ug/500ug protein lysate); Immunohistology (Frozen) (0.5-1.0ug/ml for 30 minutes at RT) Optimal dilution for a specific application should be determined.
• Localization: Predominantly cell surface with some cytoplasmic
• Positive Control: Daudi, Raji, and U266, and human lymphocytes. In normal lymph nodes and tonsils, CD20 is predominantly detected on B cells in germinal centers.
• Human Chromosomal Location: 11q12.2
• Immunogen: Lymphoblastoid cell line Daudi
• Quantity and Buffer: 0.5 ml at 200ug/ml with BSA and azide
• Preparation and Storage: Store at 2 to 8 degree C. Antibody is stable for 24 months.

References

1. Schlossman S, et al. (eds). Leukocyte Typing V, Oxford University Press, Oxford, p511-515, 1995.
2. Tedder TF and Schlossman SF. Phosphorylation of the B1 (CD20) molecule by normal and malignant human B lymphocytes. J Biol Chem 1988, 263(20):10009-10015.
3. Bubien JK et al. Transfection of the CD20 cell surface molecule into ectopic cell types generates a Ca2+ conductance found constitutively in B lymphocytes. J Cell Biol 1993, 121(5):1121-1132.
4. Tedder TF and Engel P. CD20: a regulator of cell-cycle progression of B lymphocytes. Immunol Today 1994, 15(9):450-454.
5. Kanzaki M et al. Expression of calcium-permeable cation channel CD20 accelerates progression through the G1 phase in Balb/c 3T3 cells. J Biol Chem 1995, 270(22):13099-13104.

NCBI and Uniprot Product Information

• NCBI GI #: 23110987
• NCBI GeneID: 931
• NCBI Accession #: NP_068769.2
• NCBI GenBank Nucleotide #: NM_021950.3
• UniProt Accession #: P11836; P08984; Q13963; A6NMS4
• Molecular Weight: 33-37kDa
• NCBI Official Full Name: B-lymphocyte antigen CD20
• NCBI Official Synonym Full Names: membrane-spanning 4-domains, subfamily A, member 1
• NCBI Official Symbol: MS4A1
• NCBI Official Synonym Symbols: B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
• NCBI Protein Information: B-lymphocyte antigen CD20; CD20 antigen; CD20 receptor; leukocyte surface antigen Leu-16; B-lymphocyte cell-surface antigen B1
• UniProt Protein Name: B-lymphocyte antigen CD20
• Protein Family: CD209 antigen
• UniProt Gene Name: MS4A1
• UniProt Synonym Gene Names: CD20
• UniProt Entry Name: CD20_HUMAN

NCBI Description

This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

MS4A1: This protein may be involved in the regulation of B-cell activation and proliferation. Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5); also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. Belongs to the MS4A family.

Protein type: Membrane protein, integral; Cell surface; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: extracellular space; integral to plasma membrane; external side of plasma membrane

Molecular Function: epidermal growth factor receptor binding

Biological Process: B cell proliferation; humoral immune response

Disease: Immunodeficiency, Common Variable, 5