Flow Cytometry (FC/FACS)
(Staining of normal human peripheral blood cells with CD19 antibody and 0.125 ug of Mouse IgG2b K Isotype Control (biotin) (left) or 0.125 ug of CD20 antibody (biotin) (right) followed by Streptavidin (PE). Cells in the lymphocyte gate were used for analysis.)
Mouse CD20 Monoclonal Antibody | anti-CD20 antibody
CD20 antibody (biotin)
Gene Names
MS4A1; CD20
Applications
Flow Cytometry, Functional Assay
Synonyms
CD20; Monoclonal Antibody; CD20 antibody (biotin); Monoclonal CD20; Anti-CD20; Leu-16; CD-20; B1; CD 20; MS4A2; anti-CD20 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b kappa
Clone Number
2H7
Specificity
Human
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
200 ug/ml (varies by lot)
Sequence Length
297
Applicable Applications for anti-CD20 antibody
Flow Cytometry (FC/FACS)
Application Notes
FC: <= 0.125 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Biological Significance
The 2H7 monoclonal antibody reacts with human CD20, a 33-36 kDa transmembrane protein. CD20 is expressed by developing B cells as well as mature B cells but not plasma cells. CD20 has been detected at low levels on a small subset of mature T cells. It is suggested that CD20 plays a role in B-cell activation.
Tag/Conjugate
Biotin
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Staining of normal human peripheral blood cells with CD19 antibody and 0.125 ug of Mouse IgG2b K Isotype Control (biotin) (left) or 0.125 ug of CD20 antibody (biotin) (right) followed by Streptavidin (PE). Cells in the lymphocyte gate were used for analysis.)
Flow Cytometry (FC/FACS)
(Staining of normal human peripheral blood cells with CD19 antibody and 0.125 ug of Mouse IgG2b K Isotype Control (biotin) (left) or 0.125 ug of CD20 antibody (biotin) (right) followed by Streptavidin (PE). Cells in the lymphocyte gate were used for analysis.)
Related Product Information for anti-CD20 antibody
Mouse monoclonal CD20 antibody (biotin)
Product Categories/Family for anti-CD20 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
33,056 Da
NCBI Official Full Name
CD20
NCBI Official Symbol
MS4A1
NCBI Official Synonym Symbols
CD20
NCBI Protein Information
B-lymphocyte antigen CD20
UniProt Protein Name
B-lymphocyte antigen CD20
Protein Family
UniProt Gene Name
MS4A1
UniProt Synonym Gene Names
CD20
UniProt Entry Name
CD20_CANFA
Uniprot Description
This protein may be involved in the regulation of B-cell activation and proliferation.
Research Articles on CD20
Similar Products
Product Notes
The CD20 ms4a1 (Catalog #AAA833778) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD20 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC: <= 0.125 ug/test A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Researchers should empirically determine the suitability of the CD20 ms4a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD20, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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