Mouse CD20 Monoclonal Antibody | anti-CD20 antibody

CD20 Mouse Monoclonal Antibody

Cat. Num. AAA9400046

• Gene Names: KRT20; K20; CD20; CK20; CK-20; KRT21
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: Affinity purification using immunogen.
• Synonyms: CD20; Monoclonal Antibody; CD20 Mouse Monoclonal Antibody; CK-20; CK20; cytokeratin 20; Cytokeratin-20; K1C20; K20; anti-CD20 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone Number: 2F4
• Specificity: The CD20 antibody detects endogenous CD20 proteins.
• Purity/Purification: Affinity purification using immunogen.
• Form/Format: Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 424

• Applicable Applications for anti-CD20 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: Immunohistochemistry: 1:200
• Preparation and Storage: Store at -20 degree C

Immunohistochemistry (IHC)

(IHC staining of paraffin-embedded Human tonsil tissue with CD20 mouse mAb ¨2F4 ©diluted at 1:200.)

Related Product Information for anti-CD20 antibody

CD20 is a non glycosylated protein with a molecular weight of 35 or 37 kDa depending on the degree of phosphorylation. Although not a member of the tetraspanin superfamily of cell surface receptors, it crosses the cell membrane four times. The CD20 antigen is present on human pre B lymphocytes and on B lymphocytes at all stages of maturation, except on plasma cells. Low level expression of the CD20 antigen has been detected on normal T lymphocytes. The CD20 molecule is involved in regulation of B cell differentiation, presumably via its reported function as a Ca2+ channel subunit.

Product Categories/Family for anti-CD20 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 27894337
• NCBI GeneID: 54474
• NCBI Accession #: NP_061883.1
• NCBI GenBank Nucleotide #: NM_019010.2
• UniProt Accession #: P35900; B2R6W7
• Molecular Weight: 35~37kd as determined by SDS-PAGE
• NCBI Official Full Name: keratin, type I cytoskeletal 20
• NCBI Official Synonym Full Names: keratin 20, type I
• NCBI Official Symbol: KRT20
• NCBI Official Synonym Symbols: K20; CD20; CK20; CK-20; KRT21
• NCBI Protein Information: keratin, type I cytoskeletal 20
• UniProt Protein Name: Keratin, type I cytoskeletal 20
• Protein Family: CD209 antigen
• UniProt Gene Name: KRT20
• UniProt Synonym Gene Names: CK-20; K20
• UniProt Entry Name: K1C20_HUMAN

NCBI Description

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]

Uniprot Description

K20: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: cytoplasm; intermediate filament

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: regulation of protein secretion; apoptosis; cellular response to stress; intermediate filament organization