Mouse anti-Human CD1b,c Monoclonal Antibody | anti-CD1B antibody

CD1b,c (Thymocytes) (FITC)

Cat. Num. AAA608730

• Gene Names: CD1B; R1; CD1; CD1A; MGC125990; MGC125991
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Highly Purified; Purified by Ion Exchange chromatography.
• Synonyms: CD1b; c; Monoclonal Antibody; c (Thymocytes) (FITC); Anti -CD1b; anti-CD1B antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 5J70
• Specificity: Reacts with human CD1b and CD1c.
• Purity/Purification: Highly Purified; Purified by Ion Exchange chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide. Conjugated to fluorescein isothiocyanate (FITC).

• Applicable Applications for anti-CD1B antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence and Flow Cytometry; Dilution: Flow Cytometry: 10ul labels 10e6 cells.
• Immunogen: Thymus cells, Jurkat (T cell line)
• Hybridoma: X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

Related Product Information for anti-CD1B antibody

CD1band CD1c are cell surface glycoproteins with Mr=45kD and 43kD, respectively. CD1b an CD1c are encoded by two of five CD1 genes (designated CD1a-e) which are closely linked in a cluster mapping to chromosomal region 1q22-23. CD1a-e bear homology with histocompatibility antigens. This antibody was previously designated as reacting with CD1a. This antibody was reclassified as reacting with the CD1b/c cluster at the Seventh Leukocyte Typing Workshop.

Product Categories/Family for anti-CD1B antibody

Antibodies; Abs to CD Markers

NCBI and Uniprot Product Information

• NCBI GI #: 180056
• NCBI GeneID: 910
• UniProt Accession #: P29016; Q5TDK9; Q5TDL0; Q9UMM2; Q9UN97
• NCBI Official Full Name: CD1b antigen
• NCBI Official Synonym Full Names: CD1b molecule
• NCBI Official Symbol: CD1B
• NCBI Official Synonym Symbols: R1; CD1; CD1A; MGC125990; MGC125991
• NCBI Protein Information: T-cell surface glycoprotein CD1b; OTTHUMP00000018908; OTTHUMP00000035065; CD1B antigen, b polypeptide; cortical thymocyte antigen CD1B; differentiation antigen CD1-alpha-3
• UniProt Protein Name: T-cell surface glycoprotein CD1b
• Protein Family: T-cell surface glycoprotein
• UniProt Entry Name: CD1B_HUMAN

NCBI Description

This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail, and requires vesicular acidification to bind lipid antigens. [provided by RefSeq]

Uniprot Description

CD1B: Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: cell surface; lysosomal membrane; integral to membrane; plasma membrane; endosome membrane

Molecular Function: protein binding; beta-2-microglobulin binding; exogenous lipid antigen binding; endogenous lipid antigen binding

Biological Process: antigen processing and presentation, exogenous lipid antigen via MHC class Ib; immune response