Western Blot (WB)
(Figure 1: Western blot analysis using CD1A mouse mAb against K562 (1), RAJI (2), and MOLT4 (3) cell lysate.)
Mouse anti-Human CD1A Monoclonal Antibody | anti-CD1A antibody
CD1A Antibody
Gene Names
CD1A; R4; T6; CD1; FCB6; HTA1
Reactivity
Human
Applications
Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Affinity-Chromatography
Synonyms
CD1A; Monoclonal Antibody; CD1A Antibody; CD 1a; CD1; CD1a; CD1A Antigen; CD1A antigen; a polypeptide; CD1a molecule; CD1A_HUMAN; cluster of differentiation 1 A; cortical thymocyte antigen CD1A; differentiation antigen CD1 alpha 3; epidermal dendritic cell marker CD1a; FCB 6; FCB6; HTA 1; HTA1; hTa1 thymocyte antigen; OTTHUMP00000018907; R 4; R4; T 6; T-cell surface antigen T6/Leu-6; T-cell surface glycoprotein CD1a; T6; anti-CD1A antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Specificity
CD1A antibody detects endogenous levels of total CD1A
Purity/Purification
Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
316
Applicable Applications for anti-CD1A antibody
Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes
ELISA: 1:10000
WB: 1:500-1:2000
FC/FACS: 1:200-1:400
WB: 1:500-1:2000
FC/FACS: 1:200-1:400
Immunogen
Purified recombinant fragment of human CD1A expressed in E. Coli
Subcellular Location
Cell Membrane. Endosome Membrane. Subject to intracellular trafficking between the cell membrane and endosomes. Localizes to cell surface lipid rafts.
Tissue Specificity
Expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Figure 1: Western blot analysis using CD1A mouse mAb against K562 (1), RAJI (2), and MOLT4 (3) cell lysate.)
Western Blot (WB)
(Figure 1: Western blot analysis using CD1A mouse mAb against K562 (1), RAJI (2), and MOLT4 (3) cell lysate.)
Related Product Information for anti-CD1A antibody
Description: CD1a is a non polymorphic MHC Class 1 related cell surface glycoprotein, expressed in association with Beta 2 microglobulin. CD1a is expressed by cortical thymocytes, Langerhan's cells and by interdigitating cells. CD1a is also expressed by some malignancies of T cell lineage and in histiocytosis X.
Function: Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.
Subunit Structure: Heterodimer with B2M (beta-2-microglobulin). Interacts with CD74.
Function: Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.
Subunit Structure: Heterodimer with B2M (beta-2-microglobulin). Interacts with CD74.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 37 kDa
Predicted: 38 kDa
Predicted: 38 kDa
NCBI Official Full Name
T-cell surface glycoprotein CD1a isoform 2
NCBI Official Synonym Full Names
CD1a molecule
NCBI Official Symbol
CD1A
NCBI Official Synonym Symbols
R4; T6; CD1; FCB6; HTA1
NCBI Protein Information
T-cell surface glycoprotein CD1a
UniProt Protein Name
T-cell surface glycoprotein CD1a
Protein Family
UniProt Gene Name
CD1A
UniProt Synonym Gene Names
hTa1 thymocyte antigen
NCBI Description
This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to the plasma membrane and to recycling vesicles of the early endocytic system. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
Uniprot Description
Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.
Research Articles on CD1A
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Product Notes
The CD1A cd1a (Catalog #AAA9602392) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD1A Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD1A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS). ELISA: 1:10000 WB: 1:500-1:2000 FC/FACS: 1:200-1:400. Researchers should empirically determine the suitability of the CD1A cd1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD1A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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