Mouse anti-Human CD19 Monoclonal Antibody | anti-CD19 antibody

anti-human CD19-Purified (with Antibiotics)

Cat. Num. AAA666429

• Gene Names: CD19; B4; CVID3
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Purified (with Antibiotics)
• Synonyms: CD19; Monoclonal Antibody; anti-human CD19-Purified (with Antibiotics); Monoclonal anti-human CD19; anti-CD19 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: BU12
• Purity/Purification: Purified (with Antibiotics)
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5% Gentamicin sulfate
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 92

• Applicable Applications for anti-CD19 antibody: Flow Cytometry (FC/FACS)
• Performance: Five x 105 cultured Raji cells were washed and incubated 45 minutes on ice with 80 mul of anti-CD19 antibody at 10 mug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.7 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
• Production: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5 mg/ml Gentamicin Sulfate (as a preservative).
• Group Header: anti-human CD19
• Immunogen: Human EB-4 Burkitt lymphoma cell line
• Preparation and Storage: Store at 2 - 5 degree C. Freeze/Thawing is not recommended; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-CD19 antibody

Information: Human CD19 is a B cell restricted membrane protein expressed from early stages of B cell development and associates with the antigen receptor complex. CD19 is involved in the regulation of B cell proliferation but eventually disappears from plasma cells. Antibody BU12 recognizes the CD19 molecule of about 95 kd. Antibody BU12 induces adhesion of B cells.

References

D.T. Fearon & R.H. Carter, (1995) Annu Rev Immunol 13: 127-149. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 507-511. S. Behr & F. Schriever, (1995) J Exp Med 182: 1191-1199.

NCBI and Uniprot Product Information

• NCBI GI #: 4105281
• NCBI GeneID: 930
• Molecular Weight: 61,200 Da
• NCBI Official Full Name: CD19, partial
• NCBI Official Synonym Full Names: CD19 molecule
• NCBI Official Symbol: CD19
• NCBI Official Synonym Symbols: B4; CVID3
• NCBI Protein Information: B-lymphocyte antigen CD19; differentiation antigen CD19; T-cell surface antigen Leu-12; B-lymphocyte surface antigen B4
• UniProt Protein Name: B-lymphocyte antigen CD19
• Protein Family: B-lymphocyte antigen
• UniProt Gene Name: CD19
• UniProt Entry Name: CD19_HUMAN

NCBI Description

Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008]

Uniprot Description

CD19: a cell surface molecule which assembles with the antigen receptor of B lymphocytes. A critical signal transduction molecule that regulates B lymphocyte development, activation, and differentiation. Ligation increases intracellular calcium levels and decreases the threshold for antigen receptor signaling. Germinal center formation is significantly reduced in CD19-deficient mice.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: protein complex; integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: receptor signaling protein activity

Biological Process: epidermal growth factor receptor signaling pathway; regulation of immune response; B cell receptor signaling pathway; cell surface receptor linked signal transduction; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; innate immune response; cellular defense response; positive regulation of release of sequestered calcium ion into cytosol

Disease: Immunodeficiency, Common Variable, 2; Immunodeficiency, Common Variable, 3