Mouse anti-Rat CD18 Monoclonal Antibody | anti-CD18 antibody
CD18 (Leukocyte Function Associated Antigen, LFA-1 beta2) (Biotin)
Reactivity
Rat
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry
Purity
>= 99% Purified by Affinity Chromatography. No contaminants detected. Single band by SDS-PAGE, IEP, and/or RID. The Purified monoclonal antibody was biotinylated with N-hydroxysuccinimidobiotin and then dialyzed to remove remaining free biotin.
Synonyms
CD18; Monoclonal Antibody; CD18 (Leukocyte Function Associated Antigen; LFA-1 beta2) (Biotin); anti-CD18 antibody
Host
Mouse
Reactivity
Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8.S.303
Specificity
Recognizes the rat LFA-1 (lymphocyte function associated molecule-1) beta chain (CD18; 95-100kD) which is associated with the alpha chain (CD11a; 160-70kD). Therefore, this monoclonal antibody recognizes Mac-1 and gp150/95 as well as LFA-1. Inhibits homotypic aggregation of PHA blasts.It also blocks binding of rat lymphocytes to purified rat ICAM-1.
Purity/Purification
>= 99% Purified by Affinity Chromatography. No contaminants detected. Single band by SDS-PAGE, IEP, and/or RID. The Purified monoclonal antibody was biotinylated with N-hydroxysuccinimidobiotin and then dialyzed to remove remaining free biotin.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.05% sodium azide.
Concentration
~0.5mg/ml (varies by lot)
Applicable Applications for anti-CD18 antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Adhesion Blocking
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Rat FTL-43 cells
Conjugate
Biotin
Hybridoma
Formed from the fusion of PAI mouse myeloma cells and LN cells from a BALB/c mouse
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-CD18 antibody
LFA-1 is a general leukocyte cell adhesion molecule, its activity being regulated by cell activation.
Product Categories/Family for anti-CD18 antibody
References
1. Tamatani, T. et al., Eur. J. Immunol., 21:627 - 633 (1991) 2. Tamatani, T. et al., Eur. J. Immunol., 21:855 - 858 (1991)
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Product Notes
The CD18 (Catalog #AAA6120692) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD18 (Leukocyte Function Associated Antigen, LFA-1 beta2) (Biotin) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD18 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Adhesion Blocking. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CD18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD18, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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