Mouse CD172a Monoclonal Antibody | anti-CD172a antibody

MOUSE ANTI PIG CD172a:FITC

Cat. Num. AAA215894

• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD172a; Monoclonal Antibody; MOUSE ANTI PIG CD172a:FITC; anti-CD172a antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: BL1H7
• Form/Format: FITC; Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
• Concentration: IgG concentration 0.1 mg/ml (varies by lot)

• Applicable Applications for anti-CD172a antibody: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to 1x106 cells in 100ul. ; Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10
• Perservative Stabilisers: 0.09% Sodium Azide; 1% Bovine Serum Albumin; Preparation
• Buffer Solution: Phosphate buffered saline
• Target Species: Pig
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of porcine peripheral blood mononuclear cells with Mouse anti Porcine CD172a : FITC)

Related Product Information for anti-CD172a antibody

Mouse anti Pig CD172a, clone BL1H7 is specific for porcine CD172a, a member of the signal regulatory protein (SIRP) family (Alvarez et al. 2000). Clone BL1H7 was originally clustered as SWC3 at the Third International Swine Cluster of Differentiation Workshop (Haverson et al. 2001;Thacker et al. 2001).CD172a is expressed on monocyte derived dendritic cells (MoDCs) (Facci et al. 2010) also conventional (cDCs), plasmacytoid (pDCs) DCs and blood DCs.(Facci; Jeong et al. 2010). Clone BL1H7 immunoprecipitated a single band of ~90-110kDa from preparations of biotinylated alveolar macrophages, a result confirmed by Western blotting analysis of alveolar macrophage lysates under non reducing conditions (Alvarez et al. 2000). Mouse anti Pig CD172a, clone BL1H7 has proved a useful and reliable tool for immunohistochemical analysis of routinely processed, formalin fixed, paraffin embedded porcine tissues (Domenech et al. 2003). Aberrant expression of CD172a has been noted on porcine leukemias (Sipos et al. 2006) with blast cells co-expressing lymphocytic markers CD5 and CD25 whilst expressing the Myeloid marker CD172a in a bi-phenotypic pattern as opposed to the more characteristic single population of CD172+ cells seen in normal blood PBMC (Chamorro et al. 2005).