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WB (Western Blot)
(Detection of human DDX3 by western blot. Samples: Whole cell lysate (10 ug) from HeLa, HEK293T, MCF-7, Hep-G2, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX3 antibody AAA210973 lot 3 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse DDX3 Polyclonal Antibody | anti-DDX3 antibody
Rabbit anti-DDX3 Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
DDX3, Antibody; Rabbit anti-DDX3 Antibody, Affinity Purified; DEAD box protein 3, X-chromosomal; DEAD box, X isoform; MRX102; HLP2; helicase-like protein 2; DEAD/H box-3; ATP-dependent RNA helicase DDX3X; DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked; DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked; DDX3; DDX14; DBX; CAP-Rf; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3; DEAD-box helicase 3 X-linked; anti-DDX3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-DDX3 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human DDX3 by western blot. Samples: Whole cell lysate (10 ug) from HeLa, HEK293T, MCF-7, Hep-G2, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX3 antibody AAA210973 lot 3 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human DDX3 by western blot. Samples: Whole cell lysate (10 ug) from HeLa, HEK293T, MCF-7, Hep-G2, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX3 antibody AAA210973 lot 3 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human DDX3 by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX3 antibody (AAA210973 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of human DDX3 by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX3 antibody (AAA210973 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human DDX3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDX3 antibody (AAA210973 lot 3) used for IP at 6 ug per reaction. DDX3 was also immunoprecipitated by a previous lot of this antibody (AAA210973 lot 2) and a second antibody against a different epitope of DDX3 . For blotting immunoprecipitated DDX3, AAA210973 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human DDX3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDX3 antibody (AAA210973 lot 3) used for IP at 6 ug per reaction. DDX3 was also immunoprecipitated by a previous lot of this antibody (AAA210973 lot 2) and a second antibody against a different epitope of DDX3 . For blotting immunoprecipitated DDX3, AAA210973 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of human DDX3 by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-DDX3 (AAA210973 lot 3) used at a dilution of 1:200 (1ug/ml). Detection: DAB.)
IHC (Immunohistochemistry)
(Detection of human DDX3 by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-DDX3 (AAA210973 lot 3) used at a dilution of 1:200 (1ug/ml). Detection: DAB.)
Related Product Information for anti-DDX3 antibody
DDX3 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DDX3 exhibits a variety of functions. DDX3 is a candidate tumor suppressor that suppresses tumor growth and transcriptionally activates the p21 promoter. DDX3 has been shown to play a role in translation as an eIF4E inhibitory protein that regulates translation initiation. These findings, as well as the observation that DDX3 is regulated by hepatitis C virus and is down-regulated in hepatocellular carcinoma, suggest an important role for DDX3 in growth-regulatory functions.
Product Categories/Family for anti-DDX3 antibody
NCBI and Uniprot Product Information
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Product Notes
The DDX3 ddx3x (Catalog #AAA210973) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-DDX3 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DDX3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the DDX3 ddx3x for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DDX3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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