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WB (Western Blot)
(Detection of human and mouse YB1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, A-549, MCF-7, HEK293T, U2OS, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-YB1 antibody AAA212143 (lot AAA212143-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse YB1 Polyclonal Antibody | anti-YB1 antibody
Rabbit anti-YB1 Antibody, Affinity Purified
Gene Names
YBX1; YB1; BP-8; CSDB; DBPB; YB-1; CSDA2; NSEP1; NSEP-1; MDR-NF1
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
YB1, Antibody; Rabbit anti-YB1 Antibody, Affinity Purified; NSEP-1; Y-box-binding protein 1; Y-box transcription factor; YB-1; nuclease-sensitive element-binding protein 1; NSEP1; MDR-NF1; major histocompatibility complex, class II, Y box-binding protein I; EFI-A; DNA-binding protein B; DBPB; BP-8; CSDB; CSDA2; CCAAT-binding transcription factor I subunit A; CBF-A; enhancer factor I subunit A; YB1; Nuclease-sensitive element-binding protein 1; Y-box binding protein 1; anti-YB1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-YB1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, X. laevis, Chicken, Bovine, Rabbit
Immunogen
Between 225 and 275
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse YB1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, A-549, MCF-7, HEK293T, U2OS, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-YB1 antibody AAA212143 (lot AAA212143-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human and mouse YB1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, A-549, MCF-7, HEK293T, U2OS, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-YB1 antibody AAA212143 (lot AAA212143-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human YB1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-YB1 antibody AAA212143 (lot AAA212143-2) used for IP at 6 ug per reaction. YB1 was also immunoprecipitated by a previous lot of this antibody (lot AAA212143-1) and rabbit anti-YB1 antibody For blotting immunoprecipitated YB1, AAA212143 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human YB1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-YB1 antibody AAA212143 (lot AAA212143-2) used for IP at 6 ug per reaction. YB1 was also immunoprecipitated by a previous lot of this antibody (lot AAA212143-1) and rabbit anti-YB1 antibody For blotting immunoprecipitated YB1, AAA212143 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohiostchemistry)
(Detection of mouse YB1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-YB1 (Cat. No. AAA212143 lot 2) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of mouse YB1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-YB1 (Cat. No. AAA212143 lot 2) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human YB1 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-YB1 (Cat. No. AAA212143 lot 2) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human YB1 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-YB1 (Cat. No. AAA212143 lot 2) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
Related Product Information for anti-YB1 antibody
Y-box-binding protein 1 (YB1) Regulates the transcription of numerous genes. It has been shown to promote separation of DNA strands that contain mismatches or are modified by cisplatin, and shown to have endonucleolytic activity that can introduce nicks or breaks into double-stranded DNA (in vitro). YB1 may play a role in DNA repair. It is a component of the CRD-mediated complex that promotes MYC mRNA stability [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P67809].
Product Categories/Family for anti-YB1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,924 Da
NCBI Official Full Name
nuclease-sensitive element-binding protein 1
NCBI Official Synonym Full Names
Y box binding protein 1
NCBI Official Symbol
YBX1
NCBI Official Synonym Symbols
YB1; BP-8; CSDB; DBPB; YB-1; CSDA2; NSEP1; NSEP-1; MDR-NF1
NCBI Protein Information
nuclease-sensitive element-binding protein 1; CBF-A; EFI-A; DNA-binding protein B; Y-box-binding protein 1; Y-box transcription factor; enhancer factor I subunit A; nuclease sensitive element binding protein 1; CCAAT-binding transcription factor I subunit
UniProt Protein Name
Nuclease-sensitive element-binding protein 1
UniProt Gene Name
YBX1
UniProt Synonym Gene Names
NSEP1; YB1; CBF-A; DBPB; EFI-A; YB-1
UniProt Entry Name
YBOX1_HUMAN
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Product Notes
The YB1 ybx1 (Catalog #AAA212143) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-YB1 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's YB1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the YB1 ybx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "YB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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