Rat anti-Human: No CD155 Monoclonal Antibody | anti-CD155 antibody
CD155, Mouse, mAb 3F1
Gene Names
Pvr; PVS; mE4; HVED; Taa1; CD155; Tage4; necl-5; D7Ertd458e; 3830421F03Rik
Reactivity
Human: No
Applications
Flow Cytometry, Functional Assay
Purity
Protein G
Synonyms
CD155; Monoclonal Antibody; Mouse; mAb 3F1; Poliovirus receptor; Pvr; nectin-like protein 5; Tage4; anti-CD155 antibody
Host
Rat
Reactivity
Human: No
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
3F1
Purity/Purification
Protein G
Form/Format
0.2 um filtered in PBS + 0.1% BSA
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
Concentration
100ug/ml (varies by lot)
Sequence Length
408
Applicable Applications for anti-CD155 antibody
Flow Cytometry (FC/FACS), Functional Studies (FS)
Application Notes
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
- FC: Antibody clone 3F1 stains the extracellular domain of mouse CD155. The thymocytes were blocked with 5% mouse serum before incubation with clone 3F1 for 1 hour at 4°C As positive control CD4+CD8+ thymocytes was used and as negative control CD155-/- derived cells (Ref.1).
- FS: Antibody clone 3F1 functions as antagonist. The antibody was functionally tested by blocking adhesion of Sf9 cells expressing mCD155 to RMA, CD4+ and CD8+ cells. Furthermore, antibody clone 3F1 prevents formation of mCD155/ligand complexes (Ref.2).
- Positive control: CD4+CD8+ mouse thymocytes; Negative control: CD155-/- cells.
- FC: Antibody clone 3F1 stains the extracellular domain of mouse CD155. The thymocytes were blocked with 5% mouse serum before incubation with clone 3F1 for 1 hour at 4°C As positive control CD4+CD8+ thymocytes was used and as negative control CD155-/- derived cells (Ref.1).
- FS: Antibody clone 3F1 functions as antagonist. The antibody was functionally tested by blocking adhesion of Sf9 cells expressing mCD155 to RMA, CD4+ and CD8+ cells. Furthermore, antibody clone 3F1 prevents formation of mCD155/ligand complexes (Ref.2).
- Positive control: CD4+CD8+ mouse thymocytes; Negative control: CD155-/- cells.
Immunogen
Ectodomain of recombinant His-tagged CD155
Endotoxin
0.58 EU/mg
Conjugate
None
Preparation and Storage
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Related Product Information for anti-CD155 antibody
The monoclonal antibody 3F1 recognizes mouse poliovirus receptor, also named CD155. CD155 is expressed on cells of hematopoietic origin and by endothelial vessels inside secondary lymphoid organs including HEV. Upon activation, dendritic cells, T and B cells upregulate the surface level of CD155. CD155 is an ~ 45 kDa member of a subfamily of immunoglobulin-like adhesion receptors. Nectin 1-4 are other members of this subfamily, functioning in cell-cell contact by forming heterophilic and hemophilic trans-dimers. In contrast to nectins, CD155 lacks self adhesion potential but can interact with other nectins. This way, CD155 contributes in the formation of adherens junctions between endothelial and epithelial cells. Due to this adhesive capacity, CD155 influences processes such as cell motility, migration, growth, and formation of adherens junctions. Furthermore, CD155 is involved in immunological processes such as CD8 T cell-mediated tumor rejection, and NK cell-mediated killing. Many tumor types display an aberrant up-regulation of CD155 making them vulnerable to destruction by NK or cytotoxic T cells.
In human, poliovirus, vitronectin and several other cellular receptors were identified as ligands that bind to CD155. CD96 (TACTILE) and CD226 (DNAM-1), both members of the Ig-superfamily, are other ligands of human CD155. Murine CD155 does not serve as a functional poliovirus receptor, however, murine CD155 recognizes the same panel of ligands like nectin-3, CD96, CD226 and vitronectin. CD155-deficient mice develop normally, however, a reduced humoral immune response to antigens entering the organism via the gastrointestinal tract is shown.
In human, poliovirus, vitronectin and several other cellular receptors were identified as ligands that bind to CD155. CD96 (TACTILE) and CD226 (DNAM-1), both members of the Ig-superfamily, are other ligands of human CD155. Murine CD155 does not serve as a functional poliovirus receptor, however, murine CD155 recognizes the same panel of ligands like nectin-3, CD96, CD226 and vitronectin. CD155-deficient mice develop normally, however, a reduced humoral immune response to antigens entering the organism via the gastrointestinal tract is shown.
References
1. Maier, M. et al; The adhesion receptor CD155 determines the magnitude of humoral immune responses against orally ingested antigens. Eur J Immunol 2007, 37: 2214 2. Seth, S et al; The murine pan T cell marker CD96 is an adhesion receptor for CD155 and nectin-1. Biochem Biophys Res Commun. 2007, 364: 959
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
cd155
NCBI Official Synonym Full Names
poliovirus receptor
NCBI Official Symbol
Pvr
NCBI Official Synonym Symbols
PVS; mE4; HVED; Taa1; CD155; Tage4; necl-5; D7Ertd458e; 3830421F03Rik
NCBI Protein Information
poliovirus receptor
UniProt Protein Name
Cd155
UniProt Gene Name
Pvr
Research Articles on CD155
Similar Products
Product Notes
The CD155 pvr (Catalog #AAA584713) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The CD155, Mouse, mAb 3F1 reacts with Human: No and may cross-react with other species as described in the data sheet. AAA Biotech's CD155 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Functional Studies (FS). Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. - FC: Antibody clone 3F1 stains the extracellular domain of mouse CD155. The thymocytes were blocked with 5% mouse serum before incubation with clone 3F1 for 1 hour at 4°C As positive control CD4+CD8+ thymocytes was used and as negative control CD155-/- derived cells (Ref.1). - FS: Antibody clone 3F1 functions as antagonist. The antibody was functionally tested by blocking adhesion of Sf9 cells expressing mCD155 to RMA, CD4+ and CD8+ cells. Furthermore, antibody clone 3F1 prevents formation of mCD155/ligand complexes (Ref.2). - Positive control: CD4+CD8+ mouse thymocytes; Negative control: CD155-/- cells. Researchers should empirically determine the suitability of the CD155 pvr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD155, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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