Hamster CD152 Monoclonal Antibody | anti-CD152 antibody

HAMSTER ANTI MOUSE CD152

Cat. Num. AAA219504

• Gene Names: Ctla4; Cd152; Ly-56; Ctla-4
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, Western Blot
• Synonyms: CD152; Monoclonal Antibody; HAMSTER ANTI MOUSE CD152; anti-CD152 antibody

• Host: Hamster
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 1B8
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 223

• Applicable Applications for anti-CD152 antibody: ELISA (EIA), Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. ; Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/100
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3) ; Preparation
• Immunogen: Extracellular portion of mouse CD152 fused to a mouse IgG2a.; Fusion Partners
• Target Species: Mouse
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-CD152 antibody

Hamster anti Mouse CD152 antibody, clone 1B8 recognizes mouse CD152, commonly known as CTLA-4 (cytotoxic T-lymphocyte protein 4), a structural homolog for CD28. CD152 binds the same ligands as CD28, namely CD80 (B7.1) and CD86 (B7.2), but with much higher affinity. However, while binding of CD28 enhances T cell function, binding of CD152 inhibits it. CD152 is not detected on na¯ve T-cells and is induced upon T-cell activation. In mice, lack of CD152 leads to massive lymphoproliferative disorder leading to tissue destruction and autoimmunity, suggesting a critical role for CD152 in limiting T-cell responses. CD152 has been shown to reduce IL-2 production after ligation and can inhibit T-cell expansion. This regulatory role and the high binding affinity of CD152 make it a potential therapy for autoimmune diseases such as rheumatoid arthritis. Hamster anti Mouse CD152 antibody, clone 1B8 may be used to enhance proliferation and pro-inflammatory cytokines of lymph node cells. It may also be used to enhance tumour immunity.

NCBI and Uniprot Product Information

• NCBI GI #: 31981847
• NCBI GeneID: 12477
• NCBI Accession #: NP_033973.2
• NCBI GenBank Nucleotide #: NM_009843.4
• UniProt Accession #: P09793; Q9QZZ7
• Molecular Weight: 24,993 Da
• NCBI Official Full Name: cytotoxic T-lymphocyte protein 4 isoform 1
• NCBI Official Synonym Full Names: cytotoxic T-lymphocyte-associated protein 4
• NCBI Official Symbol: Ctla4
• NCBI Official Synonym Symbols: Cd152; Ly-56; Ctla-4
• NCBI Protein Information: cytotoxic T-lymphocyte protein 4; CD152 antigen; cytotoxic T-lymphocyte-associated antigen 4
• UniProt Protein Name: Cytotoxic T-lymphocyte protein 4
• UniProt Gene Name: Ctla4
• UniProt Synonym Gene Names: Cd152; CTLA-4
• UniProt Entry Name: CTLA4_MOUSE

NCBI Description

This gene is a member of the immunoglobulin superfamily, and encodes a protein that functions as a negative regulator of T-cell responses. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2013]

Uniprot Description

CTLA-4: Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. Genetic variation in CTLA4 influences susceptibility to systemic lupus erythematosus (SLE). SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. SLE is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Genetic variations in CTLA4 may influence susceptibility to Graves disease, an autoimmune disorder associated with overactivity of the thyroid gland and hyperthyroidism. Genetic variation in CTLA4 is the cause of susceptibility to diabetes mellitus insulin-dependent type 12 (IDDM12). A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. Genetic variation in CTLA4 is the cause of susceptibility to celiac disease type 3 (CELIAC3). It is a multifactorial disorder of the small intestine that is influenced by both environmental and genetic factors. It is characterized by malabsorption resulting from inflammatory injury to the mucosa of the small intestine after the ingestion of wheat gluten or related rye and barley proteins. In its classic form, celiac disease is characterized in children by malabsorption and failure to thrive. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Immunoglobulin superfamily

Cellular Component: Golgi apparatus; membrane; perinuclear region of cytoplasm; integral to membrane; plasma membrane; clathrin-coated endocytic vesicle; external side of plasma membrane

Biological Process: B cell receptor signaling pathway; negative regulation of T cell proliferation; negative regulation of regulatory T cell differentiation; positive regulation of apoptosis; immune system process; negative regulation of immune response; immune response; negative regulation of B cell proliferation; response to DNA damage stimulus