Ctla-4 active protein

Recombinant mouse Ctla-4 protein

Cat. Num. AAA201359

• Gene Names: Ctla4; Cd152; Ly-56; Ctla-4
• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: Ctla-4; Recombinant mouse Ctla-4 protein; cytotoxic T-lymphocyte protein 4 isoform 1; Ly-5; Ctla; Cytotoxic T-lymphocyte-associated antigen 4; CTLA4; Cd152; Ly-56; Ctla4; Ctla-4 active protein

• Host: Baculovirus
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 38-161aa
• Sequence: IQVTQPSVVL ASSHGVASFP CEYSPSHNTD EVRVTVLRQT NDQMTEVCAT TFTEKNTVGF LDYPFCSGTF NESRVNLTIQ GLRAVDTGLY LCKVELMYPP PYFVGMGNGT QIYVIDPEPC PDSD

• Applicable Applications for Ctla-4 active protein: SDS-PAGE, Bioactivity
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Mouse
• Tag: hIgG-His-Tag
• Predicted N-term: Ile 38
• Bioactivity: The activity is determined by the IL-2 ELISA in a using stimulated Jurkat human acute T cell leukemia cells with Human B7-1/CD80. The ED50 range <=150ng/ml.
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

Biological Activity

(Mouse Ctla4 inhibits IL-2 secretion of the stimulated Jurkat human acute T cell leukemia cells with Human B7-1/CD80. The ED50 range )

Related Product Information for Ctla-4 active protein

Ctla-4, also known as cytotoxic T-lymphocyte protein 4 isoform 1, is a type 1 membrane protein and a memberof the immunoglobulin-superfamily. Besides, this protein contains an extra cellular domain, a transmembranedomain and a cytoplasmic tail. It is generally expressed with highest levels in lymphoid tissues. Also, it is similarto T-cell costimulatory protein CD28 since both of the molecules bind to CD80 and CD86 on antigen-presenting cells. CTLA4 and CD28 effectively regulate to T cell responses as opposing signals transmitted through tworelated cell-surface receptors. Recombinant mouse Ctla-4, fused to hIgG-His-tag at C-terminus, was expressed ininsect cell and purified by using conventional chromatography techniques.

Product Categories/Family for Ctla-4 active protein

Immunology; Proteins

References

Krummey SM., et al. (2014) J. Immunol. 192:2495-2504. Jain N., et al. (2013) Nat. Med. 19:1632-1637. Yu CR., et al. (2013) J. Immunol. 191:5036-5043.

NCBI and Uniprot Product Information

• NCBI GI #: 31981847
• NCBI GeneID: 12477
• NCBI Accession #: NP_033973.2
• NCBI GenBank Nucleotide #: NM_009843.4
• UniProt Accession #: P09793; Q9QZZ7
• Molecular Weight: 24,993 Da
• NCBI Official Full Name: cytotoxic T-lymphocyte protein 4 isoform 1
• NCBI Official Synonym Full Names: cytotoxic T-lymphocyte-associated protein 4
• NCBI Official Symbol: Ctla4
• NCBI Official Synonym Symbols: Cd152; Ly-56; Ctla-4
• NCBI Protein Information: cytotoxic T-lymphocyte protein 4; CD152 antigen; cytotoxic T-lymphocyte-associated antigen 4
• UniProt Protein Name: Cytotoxic T-lymphocyte protein 4
• UniProt Gene Name: Ctla4
• UniProt Synonym Gene Names: Cd152; CTLA-4
• UniProt Entry Name: CTLA4_MOUSE

NCBI Description

This gene is a member of the immunoglobulin superfamily, and encodes a protein that functions as a negative regulator of T-cell responses. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2013]

Uniprot Description

CTLA-4: Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. Genetic variation in CTLA4 influences susceptibility to systemic lupus erythematosus (SLE). SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. SLE is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Genetic variations in CTLA4 may influence susceptibility to Graves disease, an autoimmune disorder associated with overactivity of the thyroid gland and hyperthyroidism. Genetic variation in CTLA4 is the cause of susceptibility to diabetes mellitus insulin-dependent type 12 (IDDM12). A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. Genetic variation in CTLA4 is the cause of susceptibility to celiac disease type 3 (CELIAC3). It is a multifactorial disorder of the small intestine that is influenced by both environmental and genetic factors. It is characterized by malabsorption resulting from inflammatory injury to the mucosa of the small intestine after the ingestion of wheat gluten or related rye and barley proteins. In its classic form, celiac disease is characterized in children by malabsorption and failure to thrive. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Cellular Component: Golgi apparatus; membrane; perinuclear region of cytoplasm; plasma membrane; integral to membrane; clathrin-coated endocytic vesicle; external side of plasma membrane

Biological Process: B cell receptor signaling pathway; negative regulation of T cell proliferation; positive regulation of apoptosis; immune system process; negative regulation of regulatory T cell differentiation; negative regulation of immune response; immune response; negative regulation of B cell proliferation; response to DNA damage stimulus