Mouse CD14 Monoclonal Antibody | anti-CD14 antibody

MOUSE ANTI SHEEP CD14

Cat. Num. AAA219890

• Applications: ELISA, Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
• Synonyms: CD14; Monoclonal Antibody; MOUSE ANTI SHEEP CD14; anti-CD14 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: VPM67
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 373

• Applicable Applications for anti-CD14 antibody: ELISA (EIA), Flow cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood. ; Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/200
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3) ; Preparation
• Buffer Solution: Phosphate buffered saline
• Target Species: Sheep
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Sheep peripheral blood granulocytes stained with Mouse anti Sheep CD14 followed by Goat anti Mouse IgG:FITC (MBS235142))

Related Product Information for anti-CD14 antibody

Mouse anti Sheep CD14 monoclonal antibody, clone VPM67 recognises ovine CD14, a GPI-anchored ~55kDa membrane glycoprotein and monocyte/macrophage differentiation antigen, belonging to the lipopolysaccharide receptor family. Ovine CD14 is expressed by monocytes, macrophages and peripheral blood granulocytes (Hopkins et. al. 1996). CD14 acts as a receptor for the potent bacterial endotoxin, lipopolysaccharide (LPS), facilitated by LPS-binding protein. The binding of LPS to CD14 results in cell activation, the release of cytokines and the inflammatory response, and has been shown to upregulate the cell surface expression of adhesion molecules.Clone VPM67 has been shown to recognise CD14 in other domestic ruminant species including Water Buffalo (Bubalus bubalis) (Vimos et. al. 1996) and domestic cattle (Hopkins et. al. 1996). In addition to clone VPM67, clones VPM65 and VPM66 are also available from MyBioSource.

NCBI and Uniprot Product Information

• NCBI GI #: 41386760
• NCBI GeneID: 281048
• NCBI Accession #: NP_776433.1
• NCBI GenBank Nucleotide #: NM_174008.1
• UniProt Accession #: Q95122; Q9TVA7; A8DBS7
• Molecular Weight: 39,667 Da
• NCBI Official Full Name: monocyte differentiation antigen CD14
• NCBI Official Synonym Full Names: CD14 molecule<
• NCBI Official Symbol: CD14
• NCBI Protein Information: monocyte differentiation antigen CD14; CD14 antigen; myeloid cell-specific leucine-rich glycoprotein
• UniProt Protein Name: Monocyte differentiation antigen CD14
• Protein Family: Monocyte differentiation antigen
• UniProt Gene Name: CD14
• UniProt Entry Name: CD14_BOVIN

Uniprot Description

Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- ().