Testing Data
(Staining of pig peripheral blood granulocytes with 227403 followed by IgG (FITC) goat anti mouse)
Mouse CD14 Monoclonal Antibody | anti-CD14 antibody
CD14 (MaxLight 550)
Reactivity
Human, Porcine
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
Purity
Purified by protein G affinity chromatography from tissue culture supernatant.
Synonyms
CD14; Monoclonal Antibody; CD14 (MaxLight 550); anti-CD14 antibody
Host
Mouse
Reactivity
Human, Porcine
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
MIL2
Specificity
Recognizes porcine CD14. Species Crossreactivity: human. Demonstrates staining of both monocytes and neutrophils in peripheral blood by flow cytometry. Lymphocytes and eosinophils are negative for staining.
Purity/Purification
Purified by protein G affinity chromatography from tissue culture supernatant.
Form/Format
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight550.
Applicable Applications for anti-CD14 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
Immunogen
Porcine peripheral blood lymphocytes
Conjugate
MaxLight550
Preparation and Storage
Store at 4 degree C. Do Not Freeze.
Testing Data
(Staining of pig peripheral blood granulocytes with 227403 followed by IgG (FITC) goat anti mouse)
Testing Data
(Staining of pig peripheral blood granulocytes with 227403 followed by IgG (FITC) goat anti mouse)
Product Categories/Family for anti-CD14 antibody
References
1. Haverson, K. et al. (1994) Characterization of monoclonal antibodies specific for monocytes, macrophages and granulocytes from porcine peripheral blood and mucosal tissues. J. Imm. Methods. 170: 233-245. 2. Thacker, E. et al. (2001) Summary of workshop findings for porcine myelomonocytic markers. Vet. Immunol. Immunopathol. 80: 93-109. 3. Thorgersen, E.B. et al. (2010) CD14 inhibition efficiently attenuates early inflammatory and hemostatic responses in Escherichia coli sepsis in pigs. FASEB J. 24: 712-22. 4. Goujon, J.M. et al. (2000) Influence of cold-storage conditions on renal function of autotransplanted large pig kidneys. Kidney Int. 58: 838-50. 5. Li, Y. et al. (2014) Identification of apoptotic cells in the thymus of piglets infected with highly pathogenic porcine reproductive and respiratory syndrome virus. Virus Res. pii: S0168-1702(14)00173-7. 6. Summerfield, A. et al. (2003) Porcine peripheral blood dendritic cells and natural interferon- producing cells. Immunology. 110: 440-9. 7. Vanderheijden, N. et al. (2003) Involvement of sialoadhesin in entry of porcine reproductive and respiratory syndrome virus into porcine alveolar macrophages. J Virol. 77: 8207-15. 8. Barratt-Due, A. et al. (2011) Ornithodoros moubata Complement Inhibitor Is an Equally Effective C5 Inhibitor in Pigs and Humans. J Immunol. 187: 4913-9. 9. Hauet, T. et al. (2002) Polyethylene glycol reduces the inflammatory injury due to cold ischemia/reperfusion in autotransplanted pig kidneys. Kidney Int. 62: 654-67. 10. Kapetanovic, R. et al. (2012) Pig bone marrow-derived macrophages resemble human macrophages in their response to bacterial lipopolysaccharide. J Immunol. 188: 3382-94. 11. Thorgersen, E.B. et al. (2009) Inhibition of complement and CD14 attenuates the Escherichia coli-induced inflammatory response in porcine whole blood. Infect Immun. 77: 725-32. 12. Zelnickova, P. et al. (2007) Intracellular cytokine detection by flow cytometry in pigs: fixation, permeabilization and cell surface staining. J Immunol Methods. 327: 18-29. 13. Facci, M.R. et al. (2011) Stability of expression of reference genes in porcine peripheral blood mononuclear and dendritic cells. Vet Immunol Immunopathol. 141: 11-5. 14. Koutna', I. et al. (2012) Flow Cytometry Analysis of Intracellular Protein In: Flow Cytometry - Recent Perspectives, Schmid, I. (Ed.), ISBN: 978-953-51- 15. Facci, M.R. et al. (2010) A comparison between isolated blood dendritic cells and monocyte-derived dendritic cells in pigs. Immunology. 129: 396-405. 16. Schierack, P. et al. (2009) Effects of Bacillus cereus var. toyoi on immune parameters of pregnant sows. Vet Immunol Immunopathol.127: 26-37. 17. Lundeland, B. et al. (2011) Severe gunshot injuries in a porcine model: impact on central markers of innate immunity. Acta Anaesthesiol Scand. 55: 28-34. 18. Thorgersen, E.B. et al. (2008) Cyanobacterial LPS antagonist (CyP)-a novel and efficient inhibitor of Escherichia coli LPS-induced cytokine response in the pig. Mol Immunol. 45: 3553-7. 19. Schierack, P. et al. (2007) Bacillus cereus var. toyoi enhanced systemic immune response in piglets. Vet Immunol Immunopathol. 118: 1-11. 20. Ondrackova, P. et al. (2012) Interaction of porcine neutrophils with different strains of enterotoxigenic Escherichia coli. Vet Microbiol. 160: 108-16. 21. Ondrackova, P. et al. (2012) Phenotypic characterisation of the monocyte subpopulations in healthy adult pigs and Salmonella-infected piglets by seven-colour flow cytometry. Res Vet Sci. pii: S0034-5288(12)00256-1. 22. Vicenova, M. et al. (2014) Evaluation of in vitro and in vivo anti-inflammatory activity of biologically active phospholipids with anti-neoplastic potential in porcine model. BMC Complement Altern Med. 14: 339.
NCBI and Uniprot Product Information
UniProt Accession #
UniProt Protein Name
Monocyte differentiation antigen CD14
Protein Family
UniProt Gene Name
CD14
UniProt Entry Name
A2SW51_PIG
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Product Notes
The CD14 cd14 (Catalog #AAA6254462) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD14 (MaxLight 550) reacts with Human, Porcine and may cross-react with other species as described in the data sheet. AAA Biotech's CD14 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the CD14 cd14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD14, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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