Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Low affinity cationic amino acid transporter 2 (SLC7A2) Standard Curve.)
Rat Low Affinity Cationic Amino Acid Transporter 2 (SLC7A2) ELISA Kit | SLC7A2 elisa kit
Rat Low Affinity Cationic Amino Acid Transporter 2 (SLC7A2) ELISA Kit
Gene Names
Slc7a2; Cat2; Cat2a; Cat2b
Reactivity
Rat
Synonyms
Low Affinity Cationic Amino Acid Transporter 2 (SLC7A2); Rat Low Affinity Cationic Amino Acid Transporter 2 (SLC7A2) ELISA Kit; SLC7A2; ATRC2; CAT2; HCAT2; amino acid transporter; cationic 2; cationic amino acid transporter; y+ system; low-affinity cationic amino acid transporter-2; solute carrier family 7; member 2; SLC7A2 elisa kit
Reactivity
Rat
Specificity
Rat Low affinity cationic amino acid transporter 2 (SLC7A2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SLC7A2. No significant cross-reactivity or interference between Rat SLC7A2 and analogues was observed.
Sequence Length
658
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Low affinity cationic amino acid transporter 2 (SLC7A2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Low affinity cationic amino acid transporter 2 (SLC7A2) Standard Curve.)
Related Product Information for SLC7A2 elisa kit
Description: This Rat Low affinity cationic amino acid transporter 2 (SLC7A2) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC7A2 in samples. An antibody specific for SLC7A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLC7A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC7A2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC7A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: SLC7A2 encodes a 658-amino acid polypeptide that is 60% similar to CAT1 (ATRC1). CAT1 and CAT2 are members of the APC family of transporters, the members of which are specific for amino acids, polyamines, and choline. Northern blot analysis revealed that CAT2 is expressed as a 9.0-kb transcript in a variety of tissues. CAT2A and CAT2B are produced by alternative splicing. The deduced CAT2A and CAT2B proteins contain 657 and 658 amino acids, respectively, and both have 14 transmembrane domains and 2 putative N-glycosylation sites in extracellular loop 3. They differ from one another in 19 residues in the vicinity of transmembrane segments 8 and 9.
Background: SLC7A2 encodes a 658-amino acid polypeptide that is 60% similar to CAT1 (ATRC1). CAT1 and CAT2 are members of the APC family of transporters, the members of which are specific for amino acids, polyamines, and choline. Northern blot analysis revealed that CAT2 is expressed as a 9.0-kb transcript in a variety of tissues. CAT2A and CAT2B are produced by alternative splicing. The deduced CAT2A and CAT2B proteins contain 657 and 658 amino acids, respectively, and both have 14 transmembrane domains and 2 putative N-glycosylation sites in extracellular loop 3. They differ from one another in 19 residues in the vicinity of transmembrane segments 8 and 9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
cationic amino acid transporter 2 isoform b
NCBI Official Synonym Full Names
solute carrier family 7 member 2
NCBI Official Symbol
Slc7a2
NCBI Official Synonym Symbols
Cat2; Cat2a; Cat2b
NCBI Protein Information
cationic amino acid transporter 2
UniProt Protein Name
Cationic amino acid transporter 2
Protein Family
UniProt Gene Name
Slc7a2
UniProt Synonym Gene Names
Atrc2; Cat2; CAT-2; CAT2
NCBI Description
a glomerular cationic amino acid transporter which transports L-Arginine [RGD, Feb 2006]
Uniprot Description
Functions as permease involved in the transport of the cationic amino acids (arginine, lysine and ornithine). The affinity for its substrates differs between isoforms created by alternative splicing. May play a role in classical or alternative activation of macrophages via its role in arginine transport.
Research Articles on SLC7A2
Similar Products
Product Notes
The Rat SLC7A2 slc7a2 (Catalog #AAA9711862) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711862 ELISA Kit recognizes Rat SLC7A2. It is sometimes possible for the material contained within the vial of "Low Affinity Cationic Amino Acid Transporter 2 (SLC7A2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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