Testing Data
(Staining of human peripheral blood monocytes with MOUSE ANTI HUMAN CD14-IgG:AZIDE FREE)
Mouse CD14 Monoclonal Antibody | anti-CD14 antibody
CD14 (CD14 Antigen, Lipopolysaccharide Receptor, LPSR, Monocyte Differentiation Antigen 14, Myeloid Cell Specific Leucine Rich Glycoprotein) (FITC)
Reactivity
Human, Monkey
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation
Purity
Purified by Protein G Affinity Chromatography
Synonyms
CD14; Monoclonal Antibody; CD14 (CD14 Antigen; Lipopolysaccharide Receptor; LPSR; Monocyte Differentiation Antigen 14; Myeloid Cell Specific Leucine Rich Glycoprotein) (FITC); CD14 antigen; Lipopolysaccharide receptor; Myeloid cell specific leucine rich glycoprotein; anti-CD14 antibody
Host
Mouse
Reactivity
Human, Monkey
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
3H493
Specificity
Recognizes human CD14. Recognizes a monocyte surface antigen of 55kD found chiefly on monocytes. Gives positive staining reactions with monocytic cells, the interfollicular tissue macrophages seen under the capsule, and dendritic reticulum cells in tonsil tissue sections. Skin Langerhans cells are always negative. Also reacts with Kupffer cells and sinus lining cells on the liver. Species Crossreactivity: Cynomolgus monkey, Rhesus monkey, fish, trout.
Purity/Purification
Purified by Protein G Affinity Chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
Applicable Applications for anti-CD14 antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Immunogen
Human Thymocytes followed by peripheral blood mononuclear cells.
Conjugate
FITC
Preparation and Storage
Store at -20 degree C.
Testing Data
(Staining of human peripheral blood monocytes with MOUSE ANTI HUMAN CD14-IgG:AZIDE FREE)
Testing Data
(Staining of human peripheral blood monocytes with MOUSE ANTI HUMAN CD14-IgG:AZIDE FREE)
Product Categories/Family for anti-CD14 antibody
References
1. Hogg, N. et al. (1984) Monoclonal antibodies specific for human monocytes, granulocytes and endothelium. Immunol. 53: 753-768, 2. Linch, D.C. et al. (1984) Monoclonal antibodies differentiation between monocyte and non-monocytic varients of AML. Blood. 63: 566-573, 3. Hogg, N. and Horton, M.A. (1987) Myeloid antigens: new and previously defined clusters in Leucocyte Typing III White Cell differentiation antigens. Edited by McMichael, A.J., Beverley, P.C.L., Cobbold, S., Crumpton, M.J., Gilks, W., Gotch, F.M., Hogg, N., Horton, M., Ling, N., Mackenna, I.C.M., Mason, D.Y., Milstein, G., Speghelhalter., D. and Waldmann, H. et al, 4. Jonker, M. et al. (1989) Reactivity of mAb specific for human CD markers with Rhesus monkey leucocytes. Leucocyte Typing IV. Oxford University Press p 1058-1063, 5. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp. Anim. 49 (2): 97-110. 6. Angel, C.E. et al. (2006) Cutting edge: CD1a+ antigen-presenting cells in human dermis respond rapidly to CCR7 ligands. J. Immunol. 176: 5730-5734.7. Bournazos, S. et al. (2008) Monocyte functional responsiveness after PSGL-1-mediated platelet adhesion is dependent on platelet activation status. Arterioscler Thromb Vasc Biol. 28: 1491-8 8. Hovden, A.O. et al. (2011) Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses. BMC Immunol. 12:2. 9. Hsu, T.L. et al. (2002) Modulation of dendritic cell differentiation and maturation by decoy receptor 3. J Immunol. 168: 4846-53. 10. Karlsson, H. et al. (2002) Innate immune responses of human neonatal cells to bacteria from the normal gastrointestinal flora. Infect Immun. 70: 6688-96.
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Product Notes
The CD14 (Catalog #AAA6249507) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD14 (CD14 Antigen, Lipopolysaccharide Receptor, LPSR, Monocyte Differentiation Antigen 14, Myeloid Cell Specific Leucine Rich Glycoprotein) (FITC) reacts with Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's CD14 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the CD14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD14, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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