Mouse anti-Human CD14 Monoclonal Antibody | anti-NDUFA2 antibody

Mouse Anti-Human CD14-LE/AF

Cat. Num. AAA674220

• Gene Names: NDUFA2; B8; CD14; CIB8
• Reactivity: Human
• Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
• Synonyms: CD14; Monoclonal Antibody; Mouse Anti-Human CD14-LE/AF; Mouse Anti-Human CD14 - Low Endotoxin/Azide-Free; Mouse Anti-Human CD14; anti-NDUFA2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 61D3
• Specificity: Human CD14; CD14 is a 53-55 kDa glycosphosphatidylinositol (GPI)-anchored single chain glycoprotein. It is expressed on monocytes, macrophages, dendritic cells and Langerhans cells. It is also weakly expressed on neutrophils. CD14 appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes.1-4
• Form/Format: Low Endotoxin, Azide - Free (LE/AF)
• Sequence Length: 99

• Applicable Applications for anti-NDUFA2 antibody: Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation
• Characterization: To insure lot to lot consistency, each batch of product is tested by flow cytometry to conform to characteristics of a standard reference reagent.
• Preparation and Storage: The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. ; The fluorescein (FITC), Alexa Fluor 488 (AF488), and Alexa Fluor 647 (AF647) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL PBS/NaN3. Store at 2-8 degree C. ; The R-PE conjugate and allophycocyanin conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent; store at 2-8 degree C. ; The low endotoxin/azide-free antibody is supplied as 0.1 mg purified immunoglobulin in 0.2 mL of PBS; aliquot and freeze upon receipt. ; The Spectral Red (SPRD), R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5), R-phycoerythrin-Cyanine 7 (R-PE-CY 7), Allophycocyanin-Cyanine 5.5 (APC-CY 5.5), and Allophycocyanin-Cyanine 7 (APCCY 7) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.

References

1. Knapp, W., B. Dorken, W.R. GIlks, E.P. Rieber, R.E. Schmidt, H. Stein, A.E.G.K. Von dem Borne, eds. 1989. Leukocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, Oxford.
2. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD14 Section, Academic Press, New York, p. 169.
3. Hogg, N., S. MacDonald, M. Slusarenko, and P.C. Beverley. 1984. Immunol. 53:753.
4. Stocks, S.C., M. Albrechtsen, and M.A. Kerr. 1990. Biochem. J. 268:275.

NCBI and Uniprot Product Information

• NCBI GI #: 48146149
• NCBI GeneID: 4695
• UniProt Accession #: O43678; Q6IAY8
• Molecular Weight: 10,922 Da
• NCBI Official Full Name: CD14
• NCBI Official Synonym Full Names: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 2, 8kDa
• NCBI Official Symbol: NDUFA2
• NCBI Official Synonym Symbols: B8; CD14; CIB8
• NCBI Protein Information: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2; OTTHUMP00000159890; OTTHUMP00000223709; complex I B8 subunit; NADH-ubiquinone oxidoreductase subunit CI-B8
• UniProt Protein Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
• UniProt Gene Name: NDUFA2
• UniProt Entry Name: NDUA2_HUMAN

NCBI Description

The encoded protein is a subunit of the hydrophobic protein fraction of the NADH:ubiquinone oxidoreductase (complex 1), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane, and may be involved in regulating complex I activity or its assembly via assistance in redox processes. Mutations in this gene are associated with Leigh syndrome, an early-onset progressive neurodegenerative disorder. Alternative splicing results in multiple transcript variants.

Uniprot Description

Function: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Subunit structure: Complex I is composed of 45 different subunits.

Subcellular location: Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.