Rabbit anti-Human USP2 Polyclonal Antibody | anti-USP2 antibody

USP2, CT (L523) (Ubiquitin Carboxyl-terminal Hydrolase 2, Ubiquitin Thiolesterase 2, Ubiquitin-specific Processing Protease 2, Deubiquitinating Enzyme 2, 41kD Ubiquitin-specific Protease, UBP41) (FITC)

Cat. Num. AAA6504972

• Gene Names: USP2; USP9; UBP41
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: USP2; Polyclonal Antibody; CT (L523) (Ubiquitin Carboxyl-terminal Hydrolase 2; Ubiquitin Thiolesterase 2; Ubiquitin-specific Processing Protease 2; Deubiquitinating Enzyme 2; 41kD Ubiquitin-specific Protease; UBP41) (FITC); anti-USP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human USP2.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-USP2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human USP2 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-USP2 Antibody (C-term L523) in K562 cell line lysates (35ug/lane).USP2(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue reacted with USP2 antibody (C-term L523), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of USP2 (arrow) using rabbit polyclonal USP2 Antibody (C-term L523). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the USP2 gene.)

Product Categories/Family for anti-USP2 antibody

Antibodies; Ubiquitin

References

1. Strausberg, R.L., et al., Proc. Natl. Acad. Sci. USA 99(26):16899-16903 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 188528692
• NCBI GeneID: 9099
• NCBI Accession #: NP_004196
• NCBI GenBank Nucleotide #: NM_004205.4
• UniProt Accession #: O75604; Q8IUM2; Q8IW04; Q96MB9; Q9BQ21; B0YJB8; E9PPM2
• Molecular Weight: 45,241 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 2 isoform a
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 2
• NCBI Official Symbol: USP2
• NCBI Official Synonym Symbols: USP9; UBP41
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 2
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 2
• Protein Family: Protein
• UniProt Gene Name: USP2
• UniProt Synonym Gene Names: UBP41
• UniProt Entry Name: UBP2_HUMAN

NCBI Description

This gene encodes a member of the family of de-ubiquitinating enzymes, which belongs to the peptidase C19 superfamily. The encoded protein is a ubiquitin-specific protease which is required for TNF-alpha (tumor necrosis factor alpha) -induced NF-kB (nuclear factor kB) signaling. This protein deubiquitinates polyubiquitinated target proteins such as fatty acid synthase, murine double minute 2 (MDM2), MDM4/MDMX and cyclin D1. MDM2 and MDM4 are negative regulators of the p53 tumor suppressor and cyclin D1 is required for cell cycle G1/S transition. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Uniprot Description

USP2: Hydrolase that deubiquitinates polyubiquitinated target proteins such as MDM2, MDM4 and CCND1. Isoform 1 and isoform 4 possess both ubiquitin-specific peptidase and isopeptidase activities. Deubiquitinates MDM2 without reversing MDM2-mediated p53/TP53 ubiquitination and thus indirectly promotes p53/TP53 degradation and limits p53 activity. Has no deubiquitinase activity against p53/TP53. Prevents MDM2-mediated degradation of MDM4. Plays a role in the G1/S cell-cycle progression in normal and cancer cells. Plays a role in the regulation of myogenic differentiation of embryonic muscle cells. Homooligomer. Found in trimeric complex with MDM2 and MDM4 and UPB2. Interacts with CCND1; the interaction is direct and promotes its stabilization by antagonizing ubiquitin-dependent degradation. Interacts (via N-terminus and C- terminus) with MDM2. Interacts with MDM4. Down-regulated by cisplatin. Expressed in mesangial cells of the kidney and in different types of glomerulonephritides. Cleavage is inhibited by ubiquitin in a dosage- dependent manner. Cleavage is blocked by ubiquitin aldehyde. Belongs to the peptidase C19 family. USP2 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin-specific protease; Protease; EC 3.4.19.12

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: centrosome; perinuclear region of cytoplasm; cytoplasm; cell cortex; nucleus

Molecular Function: identical protein binding; protein binding; cyclin binding; ubiquitin protein ligase binding; metal ion binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; negative regulation of skeletal muscle development; muscle development; protein deubiquitination; protein stabilization; positive regulation of skeletal muscle development; negative regulation of transcription from RNA polymerase II promoter; positive regulation of mitotic cell cycle; cell cycle; circadian behavior; entrainment of circadian clock by photoperiod; locomotor rhythm; circadian regulation of gene expression