Western Blot (WB)
(Sample: Recombinant DISP1, Mouse.)
Rabbit anti-Mouse Dispatched Homolog 1 (DISP1) Polyclonal Antibody | anti-DISP1 antibody
Polyclonal Antibody to Dispatched Homolog 1 (DISP1)
Gene Names
Disp1; DispA; 1190008H24Rik
Reactivity
Mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Dispatched Homolog 1 (DISP1); Polyclonal Antibody; Polyclonal Antibody to Dispatched Homolog 1 (DISP1); anti-DISP1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
0.29mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-IPFPTKLQCS PFSHTLSARP GDRGPSKTHA ASAYSVDARG QKSQLEHEFY ELQPLASHSC TSSEKTTYEE PHTCSEFFNG QAKNLRMPVP AAYSSELTKS PSSEPGSALL QSCLEQDTVC HFSLNPRCNC RDAYTHLQYG LPEIHCQQMG DSLCHKCAST AGGFVQIQSS VAPLKASHQA AEGLLHPAQH MLPPGMQNSR PRNFFLHSVQ HFQAQENLGR TSTHSTDERL PRTAELSPPP SDSRSTESFQ RACCHPENNQ RRLCKSRDPG DTEGSGGTKS KVSGLPNQTD KEEKQ
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-IPFPTKLQCS PFSHTLSARP GDRGPSKTHA ASAYSVDARG QKSQLEHEFY ELQPLASHSC TSSEKTTYEE PHTCSEFFNG QAKNLRMPVP AAYSSELTKS PSSEPGSALL QSCLEQDTVC HFSLNPRCNC RDAYTHLQYG LPEIHCQQMG DSLCHKCAST AGGFVQIQSS VAPLKASHQA AEGLLHPAQH MLPPGMQNSR PRNFFLHSVQ HFQAQENLGR TSTHSTDERL PRTAELSPPP SDSRSTESFQ RACCHPENNQ RRLCKSRDPG DTEGSGGTKS KVSGLPNQTD KEEKQ
Applicable Applications for anti-DISP1 antibody
Western Blot (WB), Immunohistochemistry (IHC) - Formalin/Paraffin, Immunocytochemistry (ICC), Immunoprecipitation (IP).
Application Notes
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Organism Species
Mus musculus (Mouse)
Source
Polyclonal antibody preparation
Cross Reactivity
Mouse
Traits
Liquid
Immunogen
Recombinant DISP1 (Ile1141~Gln1435 (Accession # Q3TDN0)) expressed in E.coli (MBS2011113)
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Sample: Recombinant DISP1, Mouse.)
Western Blot (WB)
(Sample: Recombinant DISP1, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
protein dispatched homolog 1
NCBI Official Synonym Full Names
dispatched RND transporter family member 1
NCBI Official Symbol
Disp1
NCBI Official Synonym Symbols
DispA; 1190008H24Rik
NCBI Protein Information
protein dispatched homolog 1
UniProt Protein Name
Protein dispatched homolog 1
Protein Family
UniProt Gene Name
Disp1
UniProt Synonym Gene Names
Disp; Dispa; Icb; Icbins
Uniprot Description
DISP1: Functions in hedgehog (Hh) signaling. Regulates the release and extracellular accumulation of cholesterol-modified hedgehog proteins and is hence required for effective production of the Hh signal. Belongs to the dispatched family.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter
Chromosomal Location of Human Ortholog: 1 H5|1
Cellular Component: integral component of membrane; membrane
Molecular Function: peptide transporter activity
Biological Process: determination of left/right symmetry; dorsal/ventral pattern formation; embryonic pattern specification; multicellular organism development; patched ligand maturation; pattern specification process; peptide transport; smoothened signaling pathway
Research Articles on DISP1
1. Disp1 regulates growth of mammalian long bones through the control of Indian hedgehog distribution.
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Product Notes
The DISP1 disp1 (Catalog #AAA2004453) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Dispatched Homolog 1 (DISP1) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Dispatched Homolog 1 (DISP1) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) - Formalin/Paraffin, Immunocytochemistry (ICC), Immunoprecipitation (IP). . Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the DISP1 disp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-IPF PTKLQCS PFSHTLSARP GDRGPSKTHA ASAYSVDARG QKSQLEHEFY ELQPLASHSC TSSEKTTYEE PHTCSEFFNG QAKNLRMPVP AAYSSELTKS PSSEPGSALL QSCLEQDTVC HFSLNPRCNC RDAYTHLQYG LPEIHCQQMG DSLCHKCAST AGGFVQIQSS VAPLKASHQA AEGLLHPAQH MLPPGMQNSR PRNFFLHSVQ HFQAQENLGR TSTHSTDERL PRTAELSPPP SDSRSTESFQ RACCHPENNQ RRLCKSRDPG DTEGSGGTKS KVSGLPNQTD KEEKQ. It is sometimes possible for the material contained within the vial of "Dispatched Homolog 1 (DISP1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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