Rat anti-Mouse CD132 Monoclonal Antibody | anti-CD132 antibody

AF/LE Purified Anti-Mouse CD132 Monoclonal Antibody

Cat. Num. AAA2558093

• Gene Names: Il2rg; gc; p64; [g]c; CD132; gamma(c)
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation
• Synonyms: CD132; Monoclonal Antibody; AF/LE Purified Anti-Mouse CD132 Monoclonal Antibody; Cytokine receptor common subunit gamma; Il2rg; Interleukin-2 receptor subunit gamma; IL-2 receptor subunit gamma; IL-2R subunit gamma; IL-2RG; gammaC; p64; anti-CD132 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone Number: 3E12
• Form/Format: 0.2 mum filtered in PBS, pH7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or preservatives.
• Concentration: 0.5 mg/mL (varies by lot)
• Sequence Length: 369

• Applicable Applications for anti-CD132 antibody: Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
• Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is <= 2.0 ug per 10⁶ cells in 100 ul volume or 100 ul of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
• Conjugation: None (Purified antibody, Low endotoxin/Azide Free)
• Endotoxin: Endotoxin level is < 2 EU/mg (< 0.002EU/mug) as Determined by LAL gel clotting assay.
• Preparation and Storage: Store at 2-8 degree C. Do not freeze.

Related Product Information for anti-CD132 antibody

CD132 is a 64-70 kD type I transmembrane glycoprotein of the Ig superfamily, and is also known as common gamma chain (gammac) or IL-2 receptor gamma subunit. It is expressed broadly on T- and B-lymphocytes, NK cells, monocytes, and granulocytes. CD132 is an essential component of cytokine receptors for IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21. Ligand binding induces tyrosine phosphorylation and initiates signaling through a JAK/STAT pathway. CD132 mutation results in X-linked severe combined immune deficiency (XSCID).

NCBI and Uniprot Product Information

• NCBI GI #: 7305181
• NCBI GeneID: 16186
• NCBI Accession #: NP_038591.1
• NCBI GenBank Nucleotide #: NP_038591.1
• UniProt Accession #: P34902
• NCBI Official Full Name: cytokine receptor common subunit gamma isoform a
• NCBI Official Synonym Full Names: interleukin 2 receptor, gamma chain
• NCBI Official Symbol: Il2rg
• NCBI Official Synonym Symbols: gc; p64; [g]c; CD132; gamma(c)
• NCBI Protein Information: cytokine receptor common subunit gamma
• UniProt Protein Name: Cytokine receptor common subunit gamma
• UniProt Gene Name: Il2rg
• UniProt Synonym Gene Names: IL-2 receptor subunit gamma; IL-2R subunit gamma; IL-2RG
• UniProt Entry Name: IL2RG_MOUSE

NCBI Description

This gene encodes a transmembrane protein that is a common subunit of several interleukin receptor complexes. These receptors are comprised of alpha and beta subunits in addition to this gamma subunit. Signalling through this pathway in important in immune cell differentiation and function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015]

Uniprot Description

IL2RG: Common subunit for the receptors for a variety of interleukins. Defects in IL2RG are the cause of severe combined immunodeficiency X-linked T-cell-negative/B-cell-positive/NK-cell- negative (XSCID); also known as agammaglobulinemia Swiss type. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell- mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. Defects in IL2RG are the cause of X-linked combined immunodeficiency (XCID). XCID is a less severe form of X-linked immunodeficiency with a less severe degree of deficiency in cellular and humoral immunity than that seen in XSCID. Belongs to the type I cytokine receptor family. Type 5 subfamily.

Protein type: Receptor, cytokine; Membrane protein, integral

Cellular Component: cell surface; membrane; integral to membrane; external side of plasma membrane

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; interleukin-7 binding; interleukin-2 binding

Biological Process: positive regulation of T cell differentiation in the thymus; positive regulation of lymphocyte differentiation; positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation; regulation of gene expression; positive regulation of B cell differentiation