IL2RG recombinant protein

Recombinant Mouse IL2RG Protein (His & Fc tag)

Cat. Num. AAA2547173

• Gene Names: Il2rg; gc; p64; [g]c; CD132; gamma(c)
• Purity: > 92 % as determined by SDS-PAGE
• Synonyms: IL2RG; Recombinant Mouse IL2RG Protein (His & Fc tag); Il2rg; RP23-440J15.2; CD132; IL2RG recombinant protein

• Host: Human Cells
• Purity/Purification: > 92 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 369

• Application Notes: The recombinant mouse IL2RG/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 489 amino acids and has a predicted molecular mass of 56.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm IL2RG/Fc monomer is approximately 75-85 kDa due to glycosylation.
• Predicted N Terminal: Trp 23
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for IL2RG recombinant protein

Background: The common gamma chain (gammac) (or CD132), also known as interleukin-2 receptor subunit gamma or IL2RG, is a member of the type I cytokine receptor family expressed on most lymphocyte (white blood cell) populations, and its gene is found on the X-chromosome of mammals. The common gamma chain (gammac) (or IL2RG), is a cytokine receptor sub-unit that is common to the receptor complexes for at least six different interleukin receptors: IL-2, IL-4, IL-7, IL-9, IL-15 and interleukin-21 receptor. It is a component of multiple cytokine receptors that are essential for lymphocyte development and function. X-linked severe combined immunodeficiency (XSCID) is a rare and potentially fatal disease caused by mutations of IL2RG, the gene encoding IL2RG. IL2RG was demonstrated to be a component of the IL-4 receptor on the basis of chemical cross-linking data, the ability of IL2RG to augment IL-4 binding affinity. The observation that IL-2R gamma is a functional component of the IL-4 receptor, together with the finding that IL-2R gamma associates with the IL-7 receptor, begins to elucidate why deficiency of this common gamma chain (gamma c) has a profound effect on lymphoid function and development, as seen in X-linked severe combined immunodeficiency.

Description: A DNA sequence encoding the extracellular domain (Met 1-Ala 263) of mouse IL2RG (NP_038591.1) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 7305181
• NCBI GeneID: 16186
• NCBI Accession #: NP_038591.1
• NCBI GenBank Nucleotide #: NM_013563.4
• UniProt Accession #: P34902
• Molecular Weight: 42,241 Da
• NCBI Official Full Name: cytokine receptor common subunit gamma isoform a
• NCBI Official Synonym Full Names: interleukin 2 receptor, gamma chain
• NCBI Official Symbol: Il2rg
• NCBI Official Synonym Symbols: gc; p64; [g]c; CD132; gamma(c)
• NCBI Protein Information: cytokine receptor common subunit gamma
• UniProt Protein Name: Cytokine receptor common subunit gamma
• Protein Family: Cytokine receptor
• UniProt Gene Name: Il2rg
• UniProt Synonym Gene Names: IL-2 receptor subunit gamma; IL-2R subunit gamma; IL-2RG

NCBI Description

This gene encodes a transmembrane protein that is a common subunit of several interleukin receptor complexes. These receptors are comprised of alpha and beta subunits in addition to this gamma subunit. Signalling through this pathway in important in immune cell differentiation and function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015]

Uniprot Description

IL2RG: Common subunit for the receptors for a variety of interleukins. Defects in IL2RG are the cause of severe combined immunodeficiency X-linked T-cell-negative/B-cell-positive/NK-cell- negative (XSCID); also known as agammaglobulinemia Swiss type. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell- mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. Defects in IL2RG are the cause of X-linked combined immunodeficiency (XCID). XCID is a less severe form of X-linked immunodeficiency with a less severe degree of deficiency in cellular and humoral immunity than that seen in XSCID. Belongs to the type I cytokine receptor family. Type 5 subfamily.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: X D|X 43.9 cM

Cellular Component: external side of plasma membrane; membrane

Molecular Function: interleukin-2 binding

Biological Process: positive regulation of B cell differentiation; positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation; positive regulation of lymphocyte differentiation; positive regulation of T cell differentiation in the thymus; regulation of gene expression