Western Blot (WB)
(MYL12B Antibody (N-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the MYL12B antibody detected the MYL12B protein (arrow).)
Rabbit anti-Human MYL12B Polyclonal Antibody | anti-MYL12B antibody
MYL12B, NT (MYL12B, MRLC2, MYLC2B, Myosin regulatory light chain 12B, MLC-2A, Myosin regulatory light chain 2-B, smooth muscle isoform, Myosin regulatory light chain 20kD, Myosin regulatory light chain MRLC2, SHUJUN-1) (PE)
Gene Names
MYL12B; MLC-B; MRLC2
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
MYL12B; Polyclonal Antibody; NT (MYL12B; MRLC2; MYLC2B; Myosin regulatory light chain 12B; MLC-2A; Myosin regulatory light chain 2-B; smooth muscle isoform; Myosin regulatory light chain 20kD; Myosin regulatory light chain MRLC2; SHUJUN-1) (PE); SHUJUN-1; anti-MYL12B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-MYL12B antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
MYL12B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MYL12B.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(MYL12B Antibody (N-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the MYL12B antibody detected the MYL12B protein (arrow).)
Western Blot (WB)
(MYL12B Antibody (N-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the MYL12B antibody detected the MYL12B protein (arrow).)
Related Product Information for anti-MYL12B antibody
The activity of nonmuscle myosin II (see MYH9; MIM 160775) is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments (Iwasaki et al., 2001 [PubMed 11942626]).
Product Categories/Family for anti-MYL12B antibody
References
Martins-de-Souza, D., et al. Eur Arch Psychiatry Clin Neurosci 259(3):151-163(2009). Umeda, D., et al. Biochem. Biophys. Res. Commun. 333(2):628-635(2005). Li, T.B., et al. Acta Biochim. Biophys. Sin. (Shanghai) 36(6):412-418(2004). Webb, R.C. Adv Physiol Educ 27 (1-4), 201-206 (2003): Iwasaki, T., et al. Cell Struct. Funct. 26(6):677-683(2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,779 Da
NCBI Official Full Name
myosin regulatory light chain 12B
NCBI Official Synonym Full Names
myosin, light chain 12B, regulatory
NCBI Official Symbol
MYL12B
NCBI Official Synonym Symbols
MLC-B; MRLC2
NCBI Protein Information
myosin regulatory light chain 12B; MLC-2; MLC-2A; MLC20; SHUJUN-1; myosin regulatory light chain 2; myosin regulatory light chain 2-B, smooth muscle isoform; myosin regulatory light chain 20 kDa; myosin regulatory light chain MRLC2
UniProt Protein Name
Myosin regulatory light chain 12B
Protein Family
UniProt Gene Name
MYL12B
UniProt Synonym Gene Names
MRLC2; MYLC2B; MLC-2; MLC20
UniProt Entry Name
ML12B_HUMAN
NCBI Description
The activity of nonmuscle myosin II (see MYH9; MIM 160775) is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments (Iwasaki et al., 2001 [PubMed 11942626]).[supplied by OMIM, Mar 2008]
Uniprot Description
MRLC2: non-sarcomeric myosin regulatory light chain 2. Binds calcium and plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity. Phosphorylation of MLC-2 in the presence of calcium and calmodulin increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. Myosin is an hexamer of 2 heavy chains and 4 light chains.
Protein type: Contractile
Chromosomal Location of Human Ortholog: 18p11.31
Cellular Component: myosin II complex; apical part of cell; stress fiber; Z disc; cytosol
Molecular Function: protein binding; calcium ion binding
Biological Process: axon guidance; regulation of cell shape; muscle contraction; ephrin receptor signaling pathway
Research Articles on MYL12B
Similar Products
Product Notes
The MYL12B myl12b (Catalog #AAA6323318) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MYL12B, NT (MYL12B, MRLC2, MYLC2B, Myosin regulatory light chain 12B, MLC-2A, Myosin regulatory light chain 2-B, smooth muscle isoform, Myosin regulatory light chain 20kD, Myosin regulatory light chain MRLC2, SHUJUN-1) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MYL12B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MYL12B myl12b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYL12B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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