Mouse CD11c Monoclonal Antibody | anti-CD11c antibody

MOUSE ANTI HUMAN CD11c:FITC

Cat. Num. AAA224506

• Gene Names: ITGAX; CD11C; SLEB6
• Reactivity: Cynomolgus monkey; N.B. Antibody reactivity and working conditions may vary between species.
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD11c; Monoclonal Antibody; MOUSE ANTI HUMAN CD11c:FITC; anti-CD11c antibody

• Host: Mouse
• Reactivity: Cynomolgus monkey; N.B. Antibody reactivity and working conditions may vary between species.
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: BU15
• Specificity: Mouse anti Human CD11c antibody, clone Bu15 recognizes the integrin alphax subunit (ITGAX), a ~150 kDa glycoprotein also known as CD11 antigen-like family member C or Leu M5, CD11c is expressed by macrophages, monocytes, NK cells and most dendritic cells (Kohrgruber et al. 1999). CD11c is also expressed at a lower level by granulocytes.; CD11c forms a heterodimeric integral membrane protein with the integrin beta 2 chain to form the leukocyte specific integrin 'inactivated-C3b receptor 4'. CD11c interacts with a number of ligands including the G-P-R sequence in fibrinogen, ICAM-1 (Frick et al. 2005), iC3b (Sadhu et al. 2007) and Junctional adhesion molecule-like (Bazzoni et al. 2011). CD11c is involved in cell-cell interactions during the inflammatory process and is important for monocyte adhesion and chemotaxis. CD11c also acts as a signalling receptor for polysaccharide (Inqualls et al. 1995); CD11c is expressed by hairy cell leukaemia cells (Goodman et al. 2003; Nicolaou et al. 2003).
• Form/Format: Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
• Concentration: IgG concentration 0.1 mg/ml (varies by lot)
• Sequence Length: 1163

• Applicable Applications for anti-CD11c antibody: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Min. Dilution: Neat-Max. Dilution: 1/10; Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.; ; Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
• Perservative Stabilisers: 0.09% Sodium Azide; 1% Bovine Serum Albumin
• Preparation: Purified IgG prepared from tissue culture supernatant
• Buffer Solution: Phosphate buffered saline.
• Target Species: Human
• Max Ex/Em: Fluorophore: FITC; Excitation Max (nm): 490; Emission Max (nm): 525
• Preparation and Storage: Store at +4°C or at -20°C if preferred.; This product should be stored undiluted.; Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.; Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

NCBI and Uniprot Product Information

• NCBI GI #: 34452173
• NCBI GeneID: 3687
• NCBI Accession #: NP_000878.2
• NCBI GenBank Nucleotide #: NM_000887.4
• UniProt Accession #: P20702; Q8IVA6
• NCBI Official Full Name: integrin alpha-X isoform 2
• NCBI Official Synonym Full Names: integrin, alpha X (complement component 3 receptor 4 subunit)
• NCBI Official Symbol: ITGAX
• NCBI Official Synonym Symbols: CD11C; SLEB6
• NCBI Protein Information: integrin alpha-X; CD11 antigen-like family member C; integrin, alpha X (antigen CD11C (p150), alpha polypeptide); leu M5, alpha subunit; leukocyte adhesion glycoprotein p150,95 alpha chain; leukocyte adhesion receptor p150,95; leukocyte surface antigen p150,95, alpha subunit; myeloid membrane antigen, alpha subunit; p150 95 integrin alpha chain
• UniProt Protein Name: Integrin alpha-X
• UniProt Gene Name: ITGAX
• UniProt Synonym Gene Names: CD11C
• UniProt Entry Name: ITAX_HUMAN

NCBI Description

This gene encodes the integrin alpha X chain protein. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]

Uniprot Description

ITGAX: Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis. Belongs to the integrin alpha chain family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cell surface; membrane; plasma membrane; integrin complex

Molecular Function: protein binding; metal ion binding; receptor activity

Biological Process: integrin-mediated signaling pathway; heterotypic cell-cell adhesion; extracellular matrix organization and biogenesis; organ morphogenesis; blood coagulation; cell adhesion; leukocyte migration

Disease: Systemic Lupus Erythematosus