Mouse anti-Human CD105/Endoglin, Antagonistic Monoclonal Antibody | anti-CD105 antibody

Mouse Anti-Human CD105/Endoglin, Antagonistic

Cat. Num. AAA690633

• Gene Names: ENG; END; HHT1; ORW1
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Affinity chromatography
• Synonyms: CD105/Endoglin; Antagonistic; Monoclonal Antibody; Mouse Anti-Human CD105/Endoglin; Antagonistic Antibody of human Endoglin; anti-CD105 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: This antibody recognizes human endoglin in western blot.
• Purity/Purification: Affinity chromatography
• Form/Format: The antibody was purified from tissue culture supernatant by affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). The antibody is stable for twelve months when stored at -20 degree C to -70 degree C.
• Sequence Length: 625

• Applicable Applications for anti-CD105 antibody: Immunohistochemistry (IHC)
• Application Notes: Blocking TGF beta1 induced threonine/serine phosphorylation of endoglin 2. Western blotting: Yes Optimal dilutions should be determined by each laboratory for each application.
• Reconstitution: Reconstitute the antibody with 400 ul sterile PBS and the final concentration is 500 ug/ml.
• Preparation and Storage: Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.

Testing Data

Related Product Information for anti-CD105 antibody

Endoglin, also known as CD105, is a type I integral transmembrane glycoprotein and is an accessory receptor for TGF-beta superfamily ligands. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, mesenchymal stem cells and leukemic cells of lymphoid and myeloid lineages.

NCBI and Uniprot Product Information

• NCBI GI #: 4557555
• NCBI GeneID: 2022
• NCBI Accession #: NP_000109.1
• NCBI GenBank Nucleotide #: NM_000118.3
• UniProt Accession #: P17813; Q14248; Q14926; Q5T9C0
• Molecular Weight: 70,578 Da
• NCBI Official Full Name: endoglin isoform 2
• NCBI Official Synonym Full Names: endoglin
• NCBI Official Symbol: ENG
• NCBI Official Synonym Symbols: END; HHT1; ORW1
• NCBI Protein Information: endoglin; CD105 antigen
• UniProt Protein Name: Endoglin
• UniProt Gene Name: ENG
• UniProt Synonym Gene Names: END
• UniProt Entry Name: EGLN_HUMAN

NCBI Description

This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds to the beta1 and beta3 peptides with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. This gene may also be involved in preeclampsia and several types of cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]

Uniprot Description

Function: Major glycoprotein of vascular endothelium. Involved in the regulation of angiogenesis. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Acts as TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade. Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGF-beta1 signaling through SMAD3. Ref.9 Ref.11

Subunit structure: Homodimer that forms a heteromeric complex with the signaling receptors for transforming growth factor-beta: TGFBR1 and/or TGFBR2. It is able to bind TGF-beta 1, and 3 efficiently and TGF-beta 2 less efficiently. Interacts with TCTEX1D4. Interacts with ARRB2. Interacts with GDF2. Ref.6 Ref.7 Ref.9 Ref.10

Subcellular location: Membrane; Single-pass type I membrane protein.

Tissue specificity: Endoglin is restricted to endothelial cells in all tissues except bone marrow.

Involvement in disease: Telangiectasia, hereditary hemorrhagic, 1 (HHT1) [MIM:187300]: A multisystemic vascular dysplasia leading to dilation of permanent blood vessels and arteriovenous malformations of skin, mucosa, and viscera. The disease is characterized by recurrent epistaxis and gastro-intestinal hemorrhage. Visceral involvement includes arteriovenous malformations of the lung, liver, and brain.Note: The disease is caused by mutations affecting the gene represented in this entry.