Western Blot (WB)
(Western Blot Analysis: A431 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with T9154-04 (1:500 dilution of this lot). Proteins were visualized using a goat anti-rat secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein T9154-04 (~55kD).)
Rat Tubulin Monoclonal Antibody | anti-TUBA1A antibody
Tubulin (MaxLight 490)
Western Blot (WB)
(Western Blot Analysis: A431 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with T9154-04 (1:500 dilution of this lot). Proteins were visualized using a goat anti-rat secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein T9154-04 (~55kD).)
Western Blot (WB)
(Western Blot Analysis: A431 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with T9154-04 (1:500 dilution of this lot). Proteins were visualized using a goat anti-rat secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein T9154-04 (~55kD).)
NCBI and Uniprot Product Information
NCBI Description
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in this gene cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, mental retardation, and early-onset epilepsy and caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]
Uniprot Description
TUBA1A: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Expressed at a high level in fetal brain. Belongs to the tubulin family.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: 12q13.12
Cellular Component: microtubule; recycling endosome; cytoplasmic microtubule; cytosol; nucleus
Molecular Function: GTPase activity; protein domain specific binding; protein binding; GTP binding; structural constituent of cytoskeleton; structural molecule activity
Biological Process: protein polymerization; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; cell division; organelle organization and biogenesis; cytoskeleton-dependent intracellular transport; mitotic cell cycle; G2/M transition of mitotic cell cycle; microtubule-based process
Disease: Lissencephaly 3
Research Articles on TUBA1A
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Product Notes
The TUBA1A tuba1a (Catalog #AAA6245543) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Tubulin (MaxLight 490) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Tubulin can be used in a range of immunoassay formats including, but not limited to, FLISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blot (WB). Researchers should empirically determine the suitability of the TUBA1A tuba1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tubulin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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