Mouse CD103 Monoclonal Antibody | anti-CD103 antibody

MOUSE ANTI HUMAN CD103

Cat. Num. AAA213119

• Gene Names: ITGAE; CD103; HUMINAE
• Reactivity: Cynomolgus monkey, Rhesus Monkey
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
• Synonyms: CD103; Monoclonal Antibody; MOUSE ANTI HUMAN CD103; anti-CD103 antibody

• Host: Mouse
• Reactivity: Cynomolgus monkey, Rhesus Monkey
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 2G5.1
• Specificity: This item recognises the human CD103 cell surface antigen, also known as the alphaE integrin. This reacts with various subsets of human intra-epithelial lymphocytes:- 40% of lamina propria T-cells; 30% of mesenteric lymphoblasts and some lymphocytes from other mucosa. It recognises less than 0.1% bone marrow cells and less than 0.7% PBL.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 1179

• Applicable Applications for anti-CD103 antibody: Immunohistology Frozen*, Flow cytometry (FC/FACS), Immunoprecipitation (IP)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 5 x 105 cells in 100ul. ; Immunohistology - Frozen: Minimum Dilution: 1/50; Maximum Dilution: 1/100; Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections.; Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100
• Perservative Stabilisers: 0.09% Sodium Azide; 0.5% Bovine Serum Albumin; Preparation
• Immunogen: Human Intestinal lymphocyte; Histology Positive Control Tissue
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-CD103 antibody

MBS213119 recognises the human CD103 cell surface antigen, also known as the alphaE integrin. This reacts with various subsets of human intra-epithelial lymphocytes:- 40% of lamina propria T-cells; 30% of mesenteric lymphoblasts and some lymphocytes from other mucosa. It recognises less than 0.1% bone marrow cells and less than 0.7% PBL.

NCBI and Uniprot Product Information

• NCBI GI #: 148728188
• NCBI GeneID: 3682
• NCBI Accession #: NP_002199.3
• NCBI GenBank Nucleotide #: NM_002208.4
• UniProt Accession #: P38570; Q17RS6; Q9NZU9
• Molecular Weight: 130,159 Da
• NCBI Official Full Name: integrin alpha-E
• NCBI Official Synonym Full Names: integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide)
• NCBI Official Symbol: ITGAE
• NCBI Official Synonym Symbols: CD103; HUMINAE
• NCBI Protein Information: integrin alpha-E; HML-1 antigen; antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide; integrin alpha-IEL; mucosal lymphocyte 1 antigen
• UniProt Protein Name: Integrin alpha-E
• UniProt Gene Name: ITGAE
• UniProt Entry Name: ITAE_HUMAN

NCBI Description

Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain, yielding disulfide-linked heavy and light chains. In combination with the beta 7 integrin, this protein forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen. This protein is preferentially expressed in human intestinal intraepithelial lymphocytes (IEL), and in addition to a role in adhesion, it may serve as an accessory molecule for IEL activation. [provided by RefSeq, Jul 2008]

Uniprot Description

ITGAE: Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers. Belongs to the integrin alpha chain family.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: plasma membrane; integrin complex; external side of plasma membrane

Molecular Function: metal ion binding

Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; cell adhesion