Rabbit anti-Human ITGAE Polyclonal Antibody | anti-ITGAE antibody

ITGAE, CT (ITGAE, Integrin alpha-E, HML-1 antigen, Integrin alpha-IEL, Mucosal lymphocyte 1 antigen, CD103, Integrin alpha-E light chain, Integrin alpha-E heavy chain) (AP)

Cat. Num. AAA6311213

• Gene Names: ITGAE; CD103; HUMINAE
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: ITGAE; Polyclonal Antibody; CT (ITGAE; Integrin alpha-E; HML-1 antigen; Integrin alpha-IEL; Mucosal lymphocyte 1 antigen; CD103; Integrin alpha-E light chain; Integrin alpha-E heavy chain) (AP); Integrin alpha-E heavy chain; anti-ITGAE antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ITGAE.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-ITGAE antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1146-1173 from the C-terminal region of human ITGAE, UniProt Accession #P38570
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of K562 cell line lysates (35ug/lane) usingMBS646526. This demonstrates MBS646526 detected the ITGAE protein (arrow).)

Related Product Information for anti-ITGAE antibody

Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. ITGAE encodes an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain, yielding disulfide-linked heavy and light chains. In combination with the beta 7 integrin, this protein forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen. This protein is preferentially expressed in human intestinal intraepithelial lymphocytes (IEL), and in addition to a role in adhesion, it may serve as an accessory molecule for IEL activation. [provided by RefSeq].

Product Categories/Family for anti-ITGAE antibody

Antibodies; CD Markers

References

1. Silva, L.K., et al. Eur. J. Hum. Genet. 18(11):1221-1227(2010). 2. Liu, Y.H., et al. Ophthalmology 117(8):1645-1651(2010). 3. Rose, J.E., et al. Mol. Med. 16(7-8):247-253 (2010). 4. Davila, S., et al. Genes Immun. 11(3):232-238(2010). 3. Heron, M., et al. Clin. Immunol. 133(1):117-125(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 148728188
• NCBI GeneID: 3682
• NCBI Accession #: NP_002199.3
• NCBI GenBank Nucleotide #: NM_002208.4
• UniProt Accession #: P38570; Q17RS6; Q9NZU9
• Molecular Weight: 130,159 Da
• NCBI Official Full Name: integrin alpha-E
• NCBI Official Synonym Full Names: integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide)
• NCBI Official Symbol: ITGAE
• NCBI Official Synonym Symbols: CD103; HUMINAE
• NCBI Protein Information: integrin alpha-E; HML-1 antigen; antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide; integrin alpha-IEL; mucosal lymphocyte 1 antigen
• UniProt Protein Name: Integrin alpha-E
• Protein Family: Integrin
• UniProt Gene Name: ITGAE
• UniProt Entry Name: ITAE_HUMAN

NCBI Description

Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain, yielding disulfide-linked heavy and light chains. In combination with the beta 7 integrin, this protein forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen. This protein is preferentially expressed in human intestinal intraepithelial lymphocytes (IEL), and in addition to a role in adhesion, it may serve as an accessory molecule for IEL activation. [provided by RefSeq, Jul 2008]

Uniprot Description

ITGAE: Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers. Belongs to the integrin alpha chain family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: plasma membrane; integrin complex; external side of plasma membrane

Molecular Function: metal ion binding

Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; cell adhesion