Testing Data
(CCR7 in mouse splenocytes. CCR7 was|detected in immersion fixed nonstimulated|mouse splenocytes using 25ug/ml MBS632679 for 3 hours at RT. Cells were stained (red) and counterstained|(green).)
Rat anti-Mouse CCR7 Monoclonal Antibody | anti-CCR7 antibody
CCR7 (Chemokine Receptor 7, CC-CCR-7)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: Flow Cytometry: 25ug/ml; 10ul of the diluted solution labels 1-2.5x10e5 cells in a total volume 200ul.
Immunohistochemistry: 25ug/ml stains paraformaldehyde-fixed mouse splenocytes.
Neutralization: The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 for this lot of C2099-71M was determined to be approximately 2-10ug/ml in the presence of 25ng/ml of rmMIP-3beta using the BaF/3 mCCR7 cell line in a chemoattraction assay.
Testing Data
(CCR7 in mouse splenocytes. CCR7 was|detected in immersion fixed nonstimulated|mouse splenocytes using 25ug/ml MBS632679 for 3 hours at RT. Cells were stained (red) and counterstained|(green).)
Testing Data
(CCR7 in mouse splenocytes. CCR7 was|detected in immersion fixed nonstimulated|mouse splenocytes using 25ug/ml MBS632679 for 3 hours at RT. Cells were stained (red) and counterstained|(green).)
Testing Data
(Chemotaxis Induced by CCL19/MIP3beta and neutralization by MBS632679. Recombinant mouse CCL19/MIP3beta chemoattracts the BaF3 mouse proB cell line transfected with mouse CCR7 in a dose- dependent manner (orange line).)
Testing Data
(Chemotaxis Induced by CCL19/MIP3beta and neutralization by MBS632679. Recombinant mouse CCL19/MIP3beta chemoattracts the BaF3 mouse proB cell line transfected with mouse CCR7 in a dose- dependent manner (orange line).)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the G protein-coupled receptor family. This receptor was identified as a gene induced by the Epstein-Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. This receptor is expressed in various lymphoid tissues and activates B and T lymphocytes. It has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor. [provided by RefSeq]
Uniprot Description
CCR7: Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions. Belongs to the G-protein coupled receptor 1 family.
Protein type: Membrane protein, integral; Receptor, GPCR; Motility/polarity/chemotaxis; Membrane protein, multi-pass; GPCR, family 1
Chromosomal Location of Human Ortholog: 17q12-q21.2
Cellular Component: cell surface; integral to membrane; plasma membrane; intracellular; external side of plasma membrane
Molecular Function: G-protein coupled receptor activity; C-C chemokine receptor activity
Biological Process: positive regulation of cell adhesion; positive regulation of interleukin-12 production; positive regulation of dendritic cell antigen processing and presentation; positive regulation of JNK cascade; response to lipopolysaccharide; elevation of cytosolic calcium ion concentration; positive regulation of T cell receptor signaling pathway; positive regulation of humoral immune response; dendritic cell chemotaxis; establishment of T cell polarity; positive regulation of cell-matrix adhesion; inflammatory response; positive regulation of filopodium formation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of hypersensitivity; positive regulation of phosphoinositide 3-kinase activity; negative thymic T cell selection; G-protein coupled receptor protein signaling pathway; positive regulation of protein kinase B signaling cascade; myeloid dendritic cell chemotaxis; positive regulation of pseudopodium formation; positive regulation of actin filament polymerization; regulation of interferon-gamma production; positive regulation of protein kinase activity; release of sequestered calcium ion into cytosol; ruffle organization and biogenesis; regulation of interleukin-1 beta secretion; immune response
Research Articles on CCR7
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Product Notes
The CCR7 ccr7 (Catalog #AAA632679) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The CCR7 (Chemokine Receptor 7, CC-CCR-7) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CCR7 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Neutralization. Suitable for use in Flow Cytometry, Immunohistochemistry and Neutralization. Dilution: Flow Cytometry: 25ug/ml; 10ul of the diluted solution labels 1-2.5x10e5 cells in a total volume 200ul. Immunohistochemistry: 25ug/ml stains paraformaldehyde-fixed mouse splenocytes. Neutralization: The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 for this lot of C2099-71M was determined to be approximately 2-10ug/ml in the presence of 25ng/ml of rmMIP-3beta using the BaF/3 mCCR7 cell line in a chemoattraction assay. Researchers should empirically determine the suitability of the CCR7 ccr7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCR7, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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