Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-CCR7 antibody 4B12 (MBS488776) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 4B12 (MBS488776) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488776, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
Rat anti-Mouse CCR7 Monoclonal Antibody | anti-CCR7 antibody
Anti-CCR7 [4B12]
Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-CCR7 antibody 4B12 (MBS488776) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 4B12 (MBS488776) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488776, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-CCR7 antibody 4B12 (MBS488776) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 4B12 (MBS488776) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488776, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
This antibody was raised by immunizing rats with mouse CCR7 antigen.
Note on publication: The article describes the analysis of CCR7 expression during the maturation of bonemarrow-dendritic cells
NCBI and Uniprot Product Information
Uniprot Description
CCR7: Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions. Belongs to the G-protein coupled receptor 1 family.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Motility/polarity/chemotaxis; GPCR, family 1; Receptor, GPCR
Cellular Component: cell surface; external side of plasma membrane; integral to membrane; intracellular; membrane; plasma membrane
Molecular Function: C-C chemokine receptor activity; chemokine receptor activity; G-protein coupled receptor activity; protein binding; signal transducer activity
Biological Process: actin cytoskeleton organization and biogenesis; activation of JNK activity; chemotaxis; establishment of T cell polarity; G-protein coupled receptor protein signaling pathway; homeostasis of number of cells; immune response; inflammatory response; lymph node development; lymphocyte chemotaxis across high endothelial venule; myeloid dendritic cell chemotaxis; negative thymic T cell selection; positive regulation of dendritic cell antigen processing and presentation; positive regulation of filopodium formation; positive regulation of GTPase activity; positive regulation of humoral immune response; positive regulation of hypersensitivity; positive regulation of interleukin-1 beta secretion; positive regulation of interleukin-12 production; positive regulation of phagocytosis; positive regulation of receptor-mediated endocytosis; positive regulation of T cell proliferation; positive regulation of T cell receptor signaling pathway; positive regulation of T-helper 1 cell differentiation; positive regulation of tumor necrosis factor production; regulation of interferon-gamma production; regulation of interleukin-1 beta secretion; regulation of tolerance induction to self antigen; response to lipopolysaccharide; signal transduction
Research Articles on CCR7
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Product Notes
The CCR7 ccr7 (Catalog #AAA488777) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-CCR7 [4B12] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CCR7 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Functional Assay (FA). This antibody was used for flow cytometry and the staining quality can be improved by incubation at 37 degree C (Britschgi et al, 2008). This antibody is widely used to monitor the activation of dendritic cells in mice (Ohl et al, 2004; Ritter et al, 2004; Zhao et al, 2019). It was also utilized in immunoprecipitation of the CCR7 receptor protein (Ritter et al, 2004). The antibody does not interfere with ligands binding to the CCR7 receptor, which was confirmed by a transmigration assay (Ritter et al, 2004). It does not interfere with T-cell migration (Ritter et al, 2004). The antibody also is not agonistic as it does not activate the receptor (Ritter et al, 2004). Researchers should empirically determine the suitability of the CCR7 ccr7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCR7, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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