Mouse Cav-beta 2 Ca+2 Channel Monoclonal Antibody | anti-Cacnb2 antibody

Anti-Cav beta 2 Ca+2 Channel Monoclonal Antibody

Cat. Num. AAA190548

• Gene Names: Cacnb2; Cacnlb2
• Applications: Immunoblot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
• Synonyms: Cav-beta 2 Ca+2 Channel; Monoclonal Antibody; Anti-Cav beta 2 Ca+2 Channel Monoclonal Antibody; anti-Cacnb2 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: S8b-1
• Specificity: This antibody recognizes human, mouse, and rat Cavbeta2. It does not cross-react with Cav beta1, Cav beta3, or Cav beta4.
• Form/Format: 1.0mg/ml in PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.

• Applicable Applications for anti-Cacnb2 antibody: Immunoblot (IB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunofluorescence (IF)
• Application Notes: Immunoblotting: use at 1-10ug/ml. ; Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/ml. ; Immunofluorescence: use at 1-10ug/ml. ; ; These are recommended concentrations. ; User should determine optimal concentrations for their application. ; Positive control: Rat brain lysate
• Appearance: Clear Liquid
• Antigen: Synthetic peptide corresponding to aa 189-205 of rat Cavbeta2 (RSPKPSANSVTSPHSKE) (accession no.NP_446303).
• Dilution Instructions: Dilute in PBS or medium which is identical to that used in the assay system.
• Preparation and Storage: This antibody is stable for at least one (1) year at -20°C. Avoid repeated freezing and thawing.

Immunoblotting (IB)

(Adult rat brain lysate.A band of ~78kDa is detected. )

Related Product Information for anti-Cacnb2 antibody

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. Cavbeta subunits are involved in the transport of the pore-forming alpha1 subunit to the plasma membrane. They also shield an ER Retention signal on the alpha1 subunit, thereby guiding the poreforming subunit to the target membrane.

NCBI and Uniprot Product Information

• NCBI GI #: 16758716
• NCBI GeneID: 116600
• NCBI Accession #: NP_446303.1
• NCBI GenBank Nucleotide #: NM_053851.1
• UniProt Accession #: Q8VGC3; Q811Q6; Q811Q7; Q91ZJ8; Q9QUU7
• NCBI Official Full Name: voltage-dependent L-type calcium channel subunit beta-2
• NCBI Official Synonym Full Names: calcium channel, voltage-dependent, beta 2 subunit
• NCBI Official Symbol: Cacnb2
• NCBI Official Synonym Symbols: Cacnlb2
• NCBI Protein Information: voltage-dependent L-type calcium channel subunit beta-2; CAB2; calcium channel voltage-dependent subunit beta 2
• UniProt Protein Name: Voltage-dependent L-type calcium channel subunit beta-2
• Protein Family: Voltage-dependent L-type calcium channel
• UniProt Gene Name: Cacnb2
• UniProt Synonym Gene Names: Cacnlb2
• UniProt Entry Name: CACB2_RAT

NCBI Description

auxillary subunit of the L-type Ca(2+) channel [RGD]

Uniprot Description

CACNB2: The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting. Defects in CACNB2 are the cause of Brugada syndrome type 4 (BRGDA4). A heart disease characterized by the association of Brugada syndrome with shortened QT intervals. Brugada syndrome is a tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an electrocardiogram (ECG). It can cause the ventricles to beat so fast that the blood is prevented from circulating efficiently in the body. When this situation occurs (called ventricular fibrillation), the individual will faint and may die in a few minutes if the heart is not reset. Belongs to the calcium channel beta subunit family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, calcium

Cellular Component: voltage-gated calcium channel complex; sarcolemma

Molecular Function: voltage-gated calcium channel activity; protein domain specific binding; calcium channel regulator activity; high voltage-gated calcium channel activity; phosphoprotein binding; protein kinase binding

Biological Process: synaptic transmission; visual perception; calcium ion transport; positive regulation of calcium ion transport; neuromuscular junction development