Mouse anti-Human Cathepsin Z/CTSZ Monoclonal Antibody | anti-CTSZ antibody

Anti-Cathepsin Z/CTSZ Antibody, Mouse Monoclonal

Cat. Num. AAA8101375

• Gene Names: CTSZ; CTSX
• Reactivity: Human
• Applications: ELISA
• Purity: Protein A
• Synonyms: Cathepsin Z/CTSZ; Monoclonal Antibody; Anti-Cathepsin Z/CTSZ Antibody; Mouse Monoclonal; CTSZ/CTSX/Cathepsin Z Antibody; Mouse MAb; cathepsin Z; Anti-CTSX Antibody; anti-CTSZ antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 05
• Specificity: Human CTSZ/CTSX/Cathepsin Z.; No cross-reactivity in ELISA with Human CTSA, Human CTSB, Human CTSD, Human CTSL, Human CTSV, Mouse CTSZ, Human cell lysate (293 cell line)
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CTSZ antibody: ELISA (EIA)
• Application Notes: ELISA: 1:1000-1:2000
• Immunogen: Recombinant Human CTSZ/CTSX/Cathepsin Z protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CTSZ/CTSX/Cathepsin Z (rh CTSZ/CTSX/Cathepsin Z). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-CTSZ antibody

Cathepsin Z (CTSZ), also known as Cathepsin X or CATX, belongs to the C1 family of lysosomal cysteine proteases. Its gene structure and activity properties show several unique features that distinguish it clearly from other human cysteine proteases. It has a very short pro-region that shows no similarity to those of other cathepsins and a three-residue insertion motif that forms a characteristic 'mini loop'. Cathepsin Z exhibits mono-and di-peptidase activity at its C-terminus, and in contrast to cathepsin B, it does not act as an endopeptidase. It is restricted to the cells of theimmune system, predominantly monocytes, macrophages and dendritic cells. Cathepsin Z is widely expressed in human tissues, suggesting that this enzyme could be involved in the normal intracellular protein degradation taking place in all cell types. It is capable to cleave regulatory motifs at C-terminus affecting the function of targeted molecules. Cathepsin X may regulate also the maturation of dendritic cells, a process, which is crucial in the initiation of adaptive immunity. Furthermore, higher levels of Cathepsin Z are also found in tumour and immune cells of prostate and gastric carcinomas and inmacrophages of gastric mucosa, especially after infection by Helicobacter pylori. Cathepsin Z is also ubiquitously distributed in cancer cell lines and in primary tumors from different sources, suggesting that this enzyme may participate in tumor progression.

References

Santamara I, et al. (1998) Cathepsin Z, a novel human cysteine proteinase with a short propeptide domain and a unique chromosomal location. J Biol Chem. 273(27): 16816-23.Kos J, et al. (2009) The role of cathepsin X in cell signaling. Cell Adh Migr. 3(2): 164-6.Sevenich L, et al. (2010) Synergistic antitumor effects of combined cathepsin B and cathepsin Z deficiencies on breast cancer progression and metastasis in mice. Proc Natl Acad Sci U S A. 107(6): 2497-502.

NCBI and Uniprot Product Information

• NCBI GI #: 22538442
• NCBI GeneID: 1522
• NCBI Accession #: NP_001327.2
• NCBI GenBank Nucleotide #: NM_001336.3
• UniProt Accession #: Q9UBR2; O75331; Q9UQV5; Q9UQV6; B2RC40
• Molecular Weight: 33,868 Da
• NCBI Official Full Name: cathepsin Z preproprotein
• NCBI Official Synonym Full Names: cathepsin Z
• NCBI Official Symbol: CTSZ
• NCBI Official Synonym Symbols: CTSX
• NCBI Protein Information: cathepsin Z; cathepsin P; cathepsin X; cathepsin Y; cathepsin B2; cathepsin IV; cathepsin Z1; preprocathepsin P; carboxypeptidase LB; lysosomal carboxypeptidase B; cysteine-type carboxypeptidase
• UniProt Protein Name: Cathepsin Z
• Protein Family: Cathepsin
• UniProt Gene Name: CTSZ
• UniProt Entry Name: CATZ_HUMAN

NCBI Description

The protein encoded by this gene is a lysosomal cysteine proteinase and member of the peptidase C1 family. It exhibits both carboxy-monopeptidase and carboxy-dipeptidase activities. The encoded protein has also been known as cathepsin X and cathepsin P. This gene is expressed ubiquitously in cancer cell lines and primary tumors and, like other members of this family, may be involved in tumorigenesis. [provided by RefSeq, Oct 2008]

Uniprot Description

CTSZ: Exhibits carboxy-monopeptidase as well as carboxy- dipeptidase activity. Belongs to the peptidase C1 family.

Protein type: EC 3.4.18.1; Protease

Chromosomal Location of Human Ortholog: 20q13.32

Cellular Component: extracellular space; intracellular membrane-bound organelle; lysosome; endoplasmic reticulum; plasma membrane

Molecular Function: cysteine-type peptidase activity

Biological Process: cellular protein metabolic process; proteolysis; angiotensin maturation

Disease: Mycobacterium Tuberculosis, Susceptibility To