CTSZ recombinant protein
Recombinant Human CTSZ Protein (His tag)
Gene Names
CTSZ; CTSX
Purity
> 97 % as determined by SDS-PAGE
Synonyms
CTSZ; Recombinant Human CTSZ Protein (His tag); CTSX; CTSZ recombinant protein
Host
Human Cells
Purity/Purification
> 97 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH 7.4
Sequence Length
303
Application Notes
The secreted recombinant human CTSZ (pro form) consists of 291 amino acids and has a predicted molecular mass of 33 kDa. The apparent molecular mass of rhCTSZ is approximately 40 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Predicted N Terminal
Gly 24
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
SDS-PAGE
Related Product Information for CTSZ recombinant protein
Background: Cathepsin Z (CTSZ), also known as Cathepsin X or CATX, belongs to the C1 family of lysosomal cysteine proteases. Its gene structure and activity properties show several unique features that distinguish it clearly from other human cysteine proteases. It has a very short pro-region that shows no similarity to those of other cathepsins and a three-residue insertion motif that forms a characteristic 'mini loop'. Cathepsin Z exhibits mono- and di-peptidase activity at its C-terminus, and in contrast to cathepsin B, it does not act as an endopeptidase. It is restricted to the cells of theimmune system, predominantly monocytes, macrophages and dendritic cells. Cathepsin Z is widely expressed in human tissues, suggesting that this enzyme could be involved in the normal intracellular protein degradation taking place in all cell type s. It is capable to cleave regulatory motifs at C-terminus affecting the function of targeted molecules. Cathepsin X may regulate also the maturation of dendritic cells, a process, which is crucial in the initiation of adaptive immunity. Furthermore, higher levels of Cathepsin Z are also found in tumour and immune cells of prostate and gastric carcinomas and inmacrophages of gastric mucosa, especially after infection by Helicobacter pylori. Cathepsin Z is also ubiquitously distributed in cancer cell lines and in primary tumors from different sources, suggesting that this enzyme may participate in tumor progression.
Description: A DNA sequence encoding the human CTSZ (Q9UBR2) (Met 1-Val 303) was fused with a polyhistidine tag at the C-terminus.
Description: A DNA sequence encoding the human CTSZ (Q9UBR2) (Met 1-Val 303) was fused with a polyhistidine tag at the C-terminus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,868 Da
NCBI Official Full Name
cathepsin Z preproprotein
NCBI Official Synonym Full Names
cathepsin Z
NCBI Official Symbol
CTSZ
NCBI Official Synonym Symbols
CTSX
NCBI Protein Information
cathepsin Z
UniProt Protein Name
Cathepsin Z
Protein Family
UniProt Gene Name
CTSZ
NCBI Description
The protein encoded by this gene is a lysosomal cysteine proteinase and member of the peptidase C1 family. It exhibits both carboxy-monopeptidase and carboxy-dipeptidase activities. The encoded protein has also been known as cathepsin X and cathepsin P. This gene is expressed ubiquitously in cancer cell lines and primary tumors and, like other members of this family, may be involved in tumorigenesis. [provided by RefSeq, Oct 2008]
Uniprot Description
Exhibits carboxy-monopeptidase as well as carboxy-dipeptidase activity.
