Western Blot (WB)
(WB: A431 cell lysate was probed with C2069-47, anti-Catenin, beta, active (ABC). Arrow indicates beta-Catenin (92kD))
Mouse Catenin, beta, active Monoclonal Antibody | anti-CTNNB1 antibody
Catenin, beta, active (ABC) (BSA, Azide & Glycerol Free)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: Western Blot: 0.5-2ug/ml detects beta-catenin in RIPA lysates from A431 cells.
Immunohistochemisty: Detects b-catenin in mouse embryo sections.
Immunocytochemistry: Detects b-catenin in LiCl-treated 293Tcells fixed iwth methanol.
Western Blot (WB)
(WB: A431 cell lysate was probed with C2069-47, anti-Catenin, beta, active (ABC). Arrow indicates beta-Catenin (92kD))
Western Blot (WB)
(WB: A431 cell lysate was probed with C2069-47, anti-Catenin, beta, active (ABC). Arrow indicates beta-Catenin (92kD))
NCBI and Uniprot Product Information
Uniprot Description
Function: Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2
By similarity. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML
By similarity.
Subunit structure: Two separate complex-associated pools are found in the cytoplasm. The majority is present as component of an E-cadherin/ catenin adhesion complex composed of at least E-cadherin/CDH1 and beta-catenin/CTNNB1, and possibly alpha-catenin/CTNNA1; the complex is located to adherens junctions. The stable association of CTNNA1 is controversial as CTNNA1 was shown not to bind to F-actin when assembled in the complex. Alternatively, the CTNNA1-containing complex may be linked to F-actin by other proteins such as LIMA1. Another cytoplasmic pool is part of a large complex containing AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. Wnt-dependent activation of DVL antagonizes the action of GSK3B. When GSK3B activity is inhibited the complex dissociates, CTNNB1 is dephosphorylated and is no longer targeted for destruction. The stabilized protein translocates to the nucleus, where it binds TCF/LEF-1 family members, TBP, BCL9, BCL9L and possibly also RUVBL1 and CHD8. Binds CTNNBIP and EP300. CTNNB1 forms a ternary complex with LEF1 and EP300 that is disrupted by CTNNBIP1 binding
By similarity. Interacts with TAX1BP3 (via the PDZ domain); this interaction inhibits the transcriptional activity of CTNNB1. Interacts with AJAP1, BAIAP1, CARM1, CTNNA3, CXADR and PCDH11Y. Binds SLC9A3R1. Interacts with GLIS2 and SLC30A9. Interacts with XIRP1 and MUC1. Interacts with PTPRU (via the cytoplasmic juxtamembrane domain) and with EMD. Interacts with SCRIB. Interacts with TNIK. Interacts with SESTD1 and TRPC4. Interacts directly with AXIN1; the interaction is regulated by CDK2 phosphorylation of AXIN1. Interacts with CAV1. Interacts with TRPV4. The TRPV4 and CTNNB1 complex can interact with CDH1. Interacts with VCL. Interacts with PTPRJ
By similarity. Interacts with PKT7
By similarity. Interacts with FAT1 (via the cytoplasmic domain)
By similarity. Interacts with NANOS1 and NDRG2
By similarity. Interacts with NEK2, CDK2 and CDK5
By similarity. Interacts with PTK6
By similarity. Interacts with SOX7; this interaction may lead to proteasomal degradation of active CTNNB1 and thus inhibition of Wnt/beta-catenin-stimulated transcription. Identified in a complex with HINT1 and MITF. Interacts with FHIT
By similarity. The CTNNB1 and TCF4 complex interacts with PML. Interacts with FERMT2. Identified in a complex with TCF4 and FERMT2
By similarity. May interact with P-cadherin/CDH3
By similarity. Interacts with RAPGEF2. Ref.3
Subcellular location: Cytoplasm
By similarity. Cytoplasm › cytoskeleton
By similarity. Nucleus
By similarity. Cell junction › adherens junction
By similarity. Cell junction
By similarity. Cell membrane
By similarity. Cytoplasm › cytoskeleton › microtubule organizing center › centrosome
By similarity. Cytoplasm › cytoskeleton › spindle pole
By similarity. Note: Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization. The majority of beta-catenin is localized to the cell membrane. In interphase, colocalizes with CROCC between CEP250 puncta at the proximal end of centrioles, and this localization is dependent on CROCC and CEP250. In mitosis, when NEK2 activity increases, it localizes to centrosomes at spindle poles independent of CROCC. Colocalizes with CDK5 in the cell-cell contacts and plasma membrane of undifferentiated and differentiated neuroblastoma cells. Colocalized with RAPGEF2 and TJP1 at cell-cell contacts
By similarity.
Post-translational modification: Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-33. Phosphorylated by NEK2. EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding. Phosphorylated on Ser-33 and Ser-37 by HIPK2. This phosphorylation triggers proteasomal degradation. Phosphorylation at Ser-552 by AMPK promotes stabilizion of the protein, enhancing TCF/LEF-mediated transcription. Phosphorylation on Ser-191 and Ser-246 by CDK5. Phosphorylation by CDK2 regulates insulin internalization
By similarity. Phosphorylation by PTK6 at Tyr-64, Tyr-142, Tyr-331 and/or Tyr-333 with the predominant site at Tyr-64 is not essential for inhibition of transcriptional activity
By similarity.Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation
By similarity.S-nitrosylation at Cys-619 within adherens junctions promotes VEGF-induced, NO-dependent endothelial cell permeability by disrupting interaction with E-cadherin, thus mediating disassembly adherens junctions
By similarity.
Sequence similarities: Belongs to the beta-catenin family.Contains 12 ARM repeats.
Research Articles on CTNNB1
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Product Notes
The CTNNB1 ctnnb1 (Catalog #AAA608619) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Catenin, beta, active (ABC) (BSA, Azide & Glycerol Free) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Catenin, beta, active can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC). Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Dilution: Western Blot: 0.5-2ug/ml detects beta-catenin in RIPA lysates from A431 cells. Immunohistochemisty: Detects b-catenin in mouse embryo sections. Immunocytochemistry: Detects b-catenin in LiCl-treated 293Tcells fixed iwth methanol. Researchers should empirically determine the suitability of the CTNNB1 ctnnb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Catenin, beta, active, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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