Rabbit anti-Human SKP2 Polyclonal Antibody | anti-SKP2 antibody

Rabbit Polyclonal to Human SKP2

Cat. Num. AAA242697

• Gene Names: SKP2; p45; FBL1; FLB1; FBXL1
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: SKP2; Polyclonal Antibody; Rabbit Polyclonal to Human SKP2; Anti-SKP2 Antibody (aa221-425) IHC-plus; F-box/LRR-repeat protein 1; FBL1; FLB1; F-box protein Skp2; FBXL1; p45; p45skp2; Human SKP2; anti-SKP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 10 mM PBS, pH 7.4, BSA, sodium azide.
• Concentration: 0.2 mg/ml (varies by lot)
• Sequence Length: 424

• Applicable Applications for anti-SKP2 antibody: Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (10 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Recombinant protein encoding aa 221-425 of human Skp2 alpha.
• Immunogen Type: Recombinant protein
• Immunogen: SKP2 antibody was raised against recombinant protein encoding aa 221-425 of human Skp2 alpha.
• Antigen Modification: aa221-425
• Preparation and Storage: Store at 2 degree C to 8 degree C degrees. Do not freeze.

Immunohistochemistry (IHC)

(Anti-SKP2 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-SKP2 antibody IHC staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 16306595
• NCBI GeneID: 6502
• NCBI Accession #: NP_005974.2
• NCBI GenBank Nucleotide #: NM_005983.3
• UniProt Accession #: Q13309; Q8TDZ0; Q8TDZ1; Q9BV69; A8K5E0; B4DJT4
• Molecular Weight: 23,763 Da
• NCBI Official Full Name: S-phase kinase-associated protein 2 isoform 1
• NCBI Official Synonym Full Names: S-phase kinase-associated protein 2, E3 ubiquitin protein ligase
• NCBI Official Symbol: SKP2
• NCBI Official Synonym Symbols: p45; FBL1; FLB1; FBXL1
• NCBI Protein Information: S-phase kinase-associated protein 2; p45skp2; F-box/LRR-repeat protein 1; CDK2/cyclin A-associated protein p45; S-phase kinase-associated protein 2 (p45)
• UniProt Protein Name: S-phase kinase-associated protein 2
• Protein Family: S-phase kinase-associated protein
• UniProt Gene Name: SKP2
• UniProt Synonym Gene Names: FBXL1
• UniProt Entry Name: SKP2_HUMAN

NCBI Description

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class; in addition to an F-box, this protein contains 10 tandem leucine-rich repeats. This protein is an essential element of the cyclin A-CDK2 S-phase kinase. It specifically recognizes phosphorylated cyclin-dependent kinase inhibitor 1B (CDKN1B, also referred to as p27 or KIP1) predominantly in S phase and interacts with S-phase kinase-associated protein 1 (SKP1 or p19). In addition, this gene is established as a protooncogene causally involved in the pathogenesis of lymphomas. Alternative splicing of this gene generates three transcript variants encoding different isoforms. [provided by RefSeq, Jul 2011]

Uniprot Description

SKP2: an F-box protein of the SCF (SKP1-CUL1-F- box protein) E3 ubiquitin ligase system. The F-box component of the SCF complex is responsible for recognizing different substrates for ubiquitination. The SCF E3 complex mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Three alternatively-spliced isoforms have been described.

Protein type: Ubiquitin conjugating system; Cell cycle regulation

Chromosomal Location of Human Ortholog: 5p13

Cellular Component: nucleoplasm; cytoplasm; nucleolus; SCF ubiquitin ligase complex; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; ubiquitin-protein ligase activity

Biological Process: regulation of apoptosis; cell proliferation; protein polyubiquitination; regulation of cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; positive regulation of smooth muscle cell proliferation; positive regulation of estrogen receptor signaling pathway; mitotic cell cycle; G2/M transition of mitotic cell cycle; G1/S transition of mitotic cell cycle