Mouse anti-Human Caspase 8 Monoclonal Antibody | anti-CASP8 antibody

Caspase 8 Mouse Monoclonal

Cat. Num. AAA767526

• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: >=95% as determined by SDS-PAGE; Protein A+G purification
• Synonyms: Caspase 8; Monoclonal Antibody; Caspase 8 Mouse Monoclonal; anti-CASP8 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Purity/Purification: >=95% as determined by SDS-PAGE; Protein A+G purification
• Form/Format: Liquid
• Sequence Length: 479

• Applicable Applications for anti-CASP8 antibody: ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Immunogen: Caspase 8, apoptosis-related cysteine peptidase
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using MBS767526 (Caspase 8 Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer (pH9) .)

Immunofluorescence (IF)

(Immunofluorescent analysis of (-20 degree C Ethanol) fixed HeLa cells using MBS767526 (Caspase 8 Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L))

Testing Data

()

Western Blot (WB)

(HepG2 cells were subjected to SDS PAGE followed by western blot with MBS767526 (CASP8 antibody) at dilution of 1:1000)

NCBI and Uniprot Product Information

• NCBI GI #: 4583149
• Molecular Weight: 61,836 Da
• NCBI Official Full Name: caspase 8
• UniProt Protein Name: Caspase-8
• Protein Family: Caspase
• UniProt Gene Name: CASP8
• UniProt Synonym Gene Names: MCH5; CASP-8; FLICE; MACH
• UniProt Entry Name: CASP8_HUMAN

Uniprot Description

CASP8: Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death- inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 10 kDa (p10) subunit. Interacts with FADD, CFLAR and PEA15. Isoform 9 interacts at the endoplasmic reticulum with a complex containing BCAP31, BAP29, BCL2 and/or BCL2L1. Interacts with TNFAIP8L2. Interacts with human cytomegalovirus/HHV-5 protein vICA/UL36; this interaction inhibits CASP8 activation. Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle. Belongs to the peptidase C14A family. 9 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Apoptosis; EC 3.4.22.61

Chromosomal Location of Human Ortholog: 2q33-q34

Cellular Component: CD95 death-inducing signaling complex; cytoplasm; cytoskeleton; cytosol; lipid raft; microtubule organizing center; mitochondrial outer membrane; mitochondrion; neuron projection; nucleoplasm

Molecular Function: cysteine-type endopeptidase activity; cysteine-type peptidase activity; death receptor binding; peptidase activity; protein binding; protein complex binding; tumor necrosis factor receptor binding; ubiquitin protein ligase binding

Biological Process: apoptosis; B cell activation; caspase activation; cell structure disassembly during apoptosis; cell surface receptor linked signal transduction; macrophage differentiation; natural killer cell activation; negative regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of macrophage differentiation; positive regulation of proteolysis; protein heterooligomerization; proteolysis; proteolysis involved in cellular protein catabolic process; response to antibiotic; response to cobalt ion; response to cold; response to estradiol stimulus; response to ethanol; response to lipopolysaccharide; T cell activation

Disease: Breast Cancer; Caspase 8 Deficiency; Hepatocellular Carcinoma; Lung Cancer