Rat Caspase 8 ELISA Kit | CASP8 elisa kit

Rat Caspase 8 ELISA Kit

Cat. Num. AAA764040

• Gene Names: CASP8; CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8
• Reactivity: Rat
• Synonyms: Caspase 8; Rat Caspase 8 ELISA Kit; CASP8/Caspase 8/Mch5/AIS/androgen receptor/DHTRTFM/HUMARA/NR3C4KD; CASP8 elisa kit

• Reactivity: Rat
• Sequence Length: 479

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for CASP8 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Rat CASP8 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat CASP8 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat CASP8 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat CASP8 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4583149
• NCBI GeneID: 841
• Molecular Weight: 61,836 Da
• NCBI Official Full Name: caspase 8
• NCBI Official Synonym Full Names: caspase 8
• NCBI Official Symbol: CASP8
• NCBI Official Synonym Symbols: CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8
• NCBI Protein Information: caspase-8
• UniProt Protein Name: Caspase-8
• Protein Family: Caspase
• UniProt Gene Name: CASP8
• UniProt Synonym Gene Names: MCH5; CASP-8; FLICE; MACH
• UniProt Entry Name: CASP8_HUMAN

NCBI Description

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq, Jul 2008]

Uniprot Description

CASP8: Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death- inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 10 kDa (p10) subunit. Interacts with FADD, CFLAR and PEA15. Isoform 9 interacts at the endoplasmic reticulum with a complex containing BCAP31, BAP29, BCL2 and/or BCL2L1. Interacts with TNFAIP8L2. Interacts with human cytomegalovirus/HHV-5 protein vICA/UL36; this interaction inhibits CASP8 activation. Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle. Belongs to the peptidase C14A family. 9 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.61; Protease; Apoptosis

Chromosomal Location of Human Ortholog: 2q33-q34

Cellular Component: CD95 death-inducing signaling complex; cytoplasm; cytoskeleton; cytosol; lipid raft; microtubule organizing center; mitochondrial outer membrane; mitochondrion; neuron projection; Noc1p-Noc2p complex; nucleoplasm

Molecular Function: cysteine-type endopeptidase activity; cysteine-type peptidase activity; death receptor binding; peptidase activity; protein binding; protein complex binding; protein heterodimerization activity; tumor necrosis factor receptor binding; ubiquitin protein ligase binding

Biological Process: angiogenesis; apoptosis; B cell activation; caspase activation; cell structure disassembly during apoptosis; cell surface receptor linked signal transduction; heart development; innate immune response; macrophage differentiation; MyD88-independent toll-like receptor signaling pathway; natural killer cell activation; negative regulation of caspase activity; negative regulation of I-kappaB kinase/NF-kappaB cascade; neural tube formation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of macrophage differentiation; positive regulation of proteolysis; programmed cell death; protein heterooligomerization; proteolysis; proteolysis involved in cellular protein catabolic process; response to antibiotic; response to cobalt ion; response to cold; response to estradiol stimulus; response to ethanol; response to lipopolysaccharide; stimulatory C-type lectin receptor signaling pathway; T cell activation; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor signaling pathway; tumor necrosis factor-mediated signaling pathway; viral reproduction

Disease: Breast Cancer; Caspase 8 Deficiency; Hepatocellular Carcinoma; Lung Cancer