Mouse Caspase-3 (Pro and Active) Monoclonal Antibody | anti-Casp3 antibody

Caspase-3 (Pro and Active) Monoclonal Antibody

Cat. Num. AAA668032

• Gene Names: Casp3; CC3; AC-3; Lice; Yama; mldy; CPP32; SCA-1; CASP-3; CPP-32; Apopain; Caspase-3; A830040C14Rik
• Reactivity: Human (Predicted Reactivity: Mouse, Rat
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: Protein G Chromatography
• Synonyms: Caspase-3 (Pro and Active); Monoclonal Antibody; Caspase-3 (Pro and Active) Monoclonal Antibody; Casp3; Cpp32; anti-Casp3 antibody

• Host: Mouse
• Reactivity: Human (Predicted Reactivity: Mouse, Rat
• Clonality: Monoclonal
• Isotype: Mouse IgG1, Kappa
• Clone Number: ABM1C12
• Purity/Purification: Protein G Chromatography
• Form/Format: Purified; 25 ug in 50 ul/100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
• Sequence Length: 277

• Applicable Applications for anti-Casp3 antibody: Western blot analysis: 2-4 ug/ml; Immunohistochemical analysis: 5-10 Ug/ml; FACS analysis: 0.5-1 ug/10^6 cells
• Antigen: Caspase-3 (Pro and Active)
• Immunogen: Full length recombinant Caspase-3 protein was used as the immunogen for this antibody
• Preparation and Storage: Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Western Blot (WB)

(Western blot analysis of Caspase-3. Anti- Caspase-3 (Clone: ABM1C12) was used at 2 ug/ml on Ramos lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of Caspase-3 in normal human colon tissue using Caspase-3 antibody (Clone: ABM1C12) at 5 ug/ml.)

Immunohistochemistry (IHC) #2

(Immunohistochemical analysis of Caspase-3 in adenocarcinoma of rectum using Caspase-3 antibody (Clone: ABM1C12) at 5 ug/ml.)

Immunohistochemistry (IHC) #3

(Immunohistochemical analysis of Caspase-3 in normal human spleen tissue using Caspase-3 antibody (Clone: ABM1C12) at 5 ug/ml.)

Immunohistochemistry (IHC) #4

(Immunohistochemical analysis of Caspase-3 in squamous cell carcinoma of lungs using Caspase-3 antibody (Clone: ABM1C12) at 5 ug/ml.)

Immunohistochemical (IH)

(Immunohistochemical analysis of Caspase-3 in human Small intestine tissue using Caspase-3 antibdoy (Clone: ABM1C12) at 10 ug/mL/)

Immunohistochemical (IH)

(Immunohistochemical analysis of Caspase-3 in human Tonsil tissue using Caspase-3 antibody (Clone: ABM1C12) at 10 µg/ml.)

Flow Cytometric (TD

(Intracellular FLOW cytometric analysis of Caspase 3 (Clone : ABM1C12) inon Jurkat cells using 05 µg/10^6 cells of antibody. Goat anti-mouse PE conjugate was used as secondary antibody. Green represents isotope control, red represents anti-caspase 3 antibody.)

Related Product Information for anti-Casp3 antibody

Caspases are a member of the cysteine-aspartic acid protease family. Caspase-3 (31 kDa) is an executionary caspase which directly cleaves and activates poly(ADP-ribose) polymerase (PARP), sterol regulatory element binding proteins (SREBPs) or it can also interact with other caspases like caspase-6, -7 and -9. Increased levels of caspase-3 are involved in Huntington Disease-associated cell death. Caspase-3 is the principal caspase in mediating the cleavage of amyloid-beta 4A precursor protein (APP), which is related with neuronal death in Alzheimer's disease. Like other caspases, caspase-3 is also synthesized as a zymogen procaspase which is activated by specific proteolytic cleavage. High levels of caspase-3 are observed in lung, spleen, heart, liver and kidney, moderate levels in brain and skeletal muscle, and low in testis.

Product Categories/Family for anti-Casp3 antibody

Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 549806822
• NCBI GeneID: 12367
• NCBI Accession #: NP_001271338.1
• NCBI GenBank Nucleotide #: NM_001284409.1
• UniProt Accession #: P70677; O08668; Q8CHV5; Q9QWI4
• NCBI Official Full Name: caspase-3
• NCBI Official Synonym Full Names: caspase 3
• NCBI Official Symbol: Casp3
• NCBI Official Synonym Symbols: CC3; AC-3; Lice; Yama; mldy; CPP32; SCA-1; CASP-3; CPP-32; Apopain; Caspase-3; A830040C14Rik
• NCBI Protein Information: caspase-3; SREBP cleavage activity 1; caspase 3, apoptosis related cysteine protease; cysteine protease CPP32; protein Yama
• UniProt Protein Name: Caspase-3
• Protein Family: Caspase
• UniProt Gene Name: Casp3
• UniProt Synonym Gene Names: Cpp32; CASP-3; CPP-32; SCA-1
• UniProt Entry Name: CASP3_MOUSE

Uniprot Description

CASP3: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce. Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. Inhibited by isatin sulfonamides. Belongs to the peptidase C14A family.

Protein type: EC 3.4.22.56; Motility/polarity/chemotaxis; Apoptosis; Protease

Cellular Component: cytoplasm; cytosol; nucleus; lipid raft

Molecular Function: cyclin-dependent protein kinase inhibitor activity; peptidase activity; phospholipase A2 activator activity; protein binding; protease binding; hydrolase activity; cysteine-type endopeptidase activity; death receptor binding; protein complex binding; aspartic-type endopeptidase activity; cysteine-type peptidase activity

Biological Process: positive regulation of catalytic activity; apoptosis; positive regulation of apoptosis; heart development; negative regulation of activated T cell proliferation; negative regulation of B cell proliferation; proteolysis; negative regulation of cell cycle; neuron differentiation; learning and/or memory; sensory perception of sound; B cell homeostasis; positive regulation of neuron apoptosis; response to wounding; response to glucose stimulus; erythrocyte differentiation; T cell homeostasis; response to UV; release of cytochrome c from mitochondria; cell fate commitment; negative regulation of cyclin-dependent protein kinase activity; keratinocyte differentiation; neuron apoptosis; induction of apoptosis via death domain receptors; response to hydrogen peroxide; protein processing; induction of apoptosis by oxidative stress; response to DNA damage stimulus; negative regulation of apoptosis