Immunofluorescence (IF)
(MBS9610419 staining Hela cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% TritonX-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9610419 1:200)and mouse anti-beta tubulin Ab(MBS576566 1:200) for 1 hour at37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L)Ab(Red) and an AlexaFluor488 conjugated goat anti-mouseIgG(H+L) Ab(Green) were used as the secondary antibody.)
Rabbit NEK2 Polyclonal Antibody | anti-NEK2 antibody
Phospho-NEK2 (Ser171) Antibody
The optimal dilutions should be determined by the end user.
IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Immunofluorescence (IF)
(MBS9610419 staining Hela cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% TritonX-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9610419 1:200)and mouse anti-beta tubulin Ab(MBS576566 1:200) for 1 hour at37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L)Ab(Red) and an AlexaFluor488 conjugated goat anti-mouseIgG(H+L) Ab(Green) were used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9610419 staining Hela cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% TritonX-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9610419 1:200)and mouse anti-beta tubulin Ab(MBS576566 1:200) for 1 hour at37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L)Ab(Red) and an AlexaFluor488 conjugated goat anti-mouseIgG(H+L) Ab(Green) were used as the secondary antibody.)
Immunohistochemistry (IHC)
(At 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Immunohistochemistry (IHC)
(At 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Western Blot (WB)
(Western blot analysis of extracts from K562, using Phospho-NEK2(Ser171) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from K562, using Phospho-NEK2(Ser171) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene. [provided by RefSeq, Feb 2011]
Uniprot Description
NEK2: a protein kinase of the NEK family. Accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins. Phosphorylation of Hec1 by Nek2 is essential for faithful chromosome segregation
Protein type: Protein kinase, Other; Kinase, protein; EC 2.7.11.1; Nucleolus; Protein kinase, Ser/Thr (non-receptor); Other group; NEK family
Chromosomal Location of Human Ortholog: 1q32.3
Cellular Component: kinetochore; spindle pole; centrosome; microtubule; intercellular bridge; condensed nuclear chromosome; protein complex; cytoplasm; nucleolus; midbody; nucleus; cytosol
Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; protein phosphatase binding; ATP binding; protein kinase activity
Biological Process: mitosis; blastocyst development; regulation of attachment of spindle microtubules to kinetochore; mitotic sister chromatid segregation; organelle organization and biogenesis; protein amino acid autophosphorylation; regulation of mitotic centrosome separation; centrosome separation; spindle assembly; protein amino acid phosphorylation; negative regulation of DNA binding; chromosome segregation; meiotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; regulation of mitosis; mitotic cell cycle; G2/M transition of mitotic cell cycle
Disease: Retinitis Pigmentosa 67
Research Articles on NEK2
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Product Notes
The NEK2 nek2 (Catalog #AAA9610419) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-NEK2 (Ser171) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NEK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA), Immunofluorescence (IF)/Immunocytochemistry (ICC). WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000 The optimal dilutions should be determined by the end user. IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. Researchers should empirically determine the suitability of the NEK2 nek2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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