Rabbit anti-Human Carboxypeptidase E/CPE Monoclonal Antibody | anti-CPE antibody

Recombinant Anti-Carboxypeptidase E/CPE Antibody, Rabbit Monoclonal

Cat. Num. AAA8100597

• Reactivity: Human
• Applications: ELISA
• Purity: Protein A
• Synonyms: Carboxypeptidase E/CPE; Monoclonal Antibody; Recombinant Anti-Carboxypeptidase E/CPE Antibody; Rabbit Monoclonal; Carboxypeptidase E/CPE Antibody; Rabbit MAb; carboxypeptidase E; anti-CPE antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 1
• Specificity: Human CPE/Carboxypeptidase E.; No cross-reactivity in ELISA with Human Carboxypeptidase-A1/CPA1, Human Carboxypeptidase-A2/CPA2, Human Carboxypeptidase-B1/CPB1
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CPE antibody: ELISA (EIA)
• Application Notes: ELISA: 1:5000-1:10,000
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Human CPE protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human CPE (rhCPE).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-CPE antibody

Carboxypeptidase E (CPE), also known as Carboxypeptidase H, is a peripheral membrane protein and a zinc metallocarboxypeptidase, and the conversion of proCPE into CPE occurs primarily in secretory vesicles. The active form of CPE cleaves C-terminal amino acid residues of the peptide, and is thus involved in the biosynthesis of peptide hormones and neurotransmitters including insulin, enkephalin, etc. The enzymatic activity is enhanced by millimolar concentrations of Co2+. It has also been proposed that membrane-associated carboxypeptidase E acts as a sorting receptor for targeting regulated secretory proteins which are mostly prohormones and neuropeptides in the trans-Golgi network of the pituitary and in secretory granules into the secretory pathway.Its interaction with glycosphingolipid-cholesterol rafts at the TGN facilitates the targeting. Mutations in this gene are implicated in type II diabetes due to impaired glucose clearance and insulin resistance.

References

Manser, E. et al., 1990, Biochem. J. 267: 517-525. Cool, D.R. et al., 1997, Cell. 88: 73-83. Song, L. and Fricker, L. 1995, J. Neurochem. 65: 444-453. Dhanvantari, S. et al., 2000, J. Biol. Chem. 275: 29887-29893. Jeffrey, K.D. et al., 2008, Proc. Natl. Acad. Sci. U.S.A. 105: 8452-8457

NCBI and Uniprot Product Information

• NCBI GI #: 4503009
• NCBI GeneID: 1363
• NCBI Accession #: NP_001864.1
• NCBI GenBank Nucleotide #: NM_001873.2
• UniProt Accession #: P16870; Q9UIU9; A8K4N1; B3KR42; B4DFN4; D3DP33
• Molecular Weight: 53,151 Da
• NCBI Official Full Name: carboxypeptidase E preproprotein
• NCBI Official Synonym Full Names: carboxypeptidase E
• NCBI Official Symbol: CPE
• NCBI Protein Information: carboxypeptidase E; CPH; carboxypeptidase H; enkephalin convertase; prohormone-processing carboxypeptidase; insulin granule-associated carboxypeptidase; cobalt-stimulated chromaffin granule carboxypeptidase
• UniProt Protein Name: Carboxypeptidase E
• Protein Family: Carboxypeptidase
• UniProt Gene Name: CPE
• UniProt Synonym Gene Names: CPE; CPH
• UniProt Entry Name: CBPE_HUMAN

NCBI Description

This gene encodes a carboxypeptidase that cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. It is a peripheral membrane protein. The protein specifically binds regulated secretory pathway proteins, including prohormones, but not constitutively secreted proteins. Mutations in this gene are implicated in type II diabetes. [provided by RefSeq, Jul 2008]

Uniprot Description

CPE: Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Protease; EC 3.4.17.10; Secreted

Chromosomal Location of Human Ortholog: 4q32.3

Cellular Component: Golgi apparatus; secretory granule membrane; plasma membrane; nucleus

Molecular Function: carboxypeptidase activity; zinc ion binding; neurexin binding; cell adhesion molecule binding; metallocarboxypeptidase activity

Biological Process: insulin processing; cellular protein metabolic process; metabolic process; neuropeptide signaling pathway; protein modification process; proteolysis