Mouse CaMKII Monoclonal Antibody | anti-CaMKII antibody

CaMKII (Alpha-Specific) Antibody

Cat. Num. AAA8001881

• Gene Names: Camk2a; CaMKII; R74975; mKIAA0968
• Reactivity: Human, Mouse, Rat, Bovine
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, ELISA, immunoassay, Radioimmunoassay
• Purity: Protein G Purified
• Synonyms: CaMKII; Monoclonal Antibody; CaMKII (Alpha-Specific) Antibody; Mouse Anti-Rat CaMKII (Alpha-Specific) Monoclonal IgG1; CaMKII Antibody; Clone 6G9; CamK2 Antibody; CamK2A Antibody; CamK2B Antibody; CamK2D Antibody; CamK2G Antibody; CAMKA Antibody; anti-CaMKII antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Bovine
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 6G9
• Specificity: Detects ~50-60kDa. Recognizes both phosphorylated and non-phosphorylated forms.
• Purity/Purification: Protein G Purified
• Form/Format: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 478

• Applicable Applications for anti-CaMKII antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA (EIA), Radioimmunoassay (RIA)
• Application Notes: WB: 1:10000; IHC: 1:2000; ICC/IF: 1:50
• Immunogen: Partially purified rat CaMKII
• Immunogen Species: Rat
• Conjugate: Unconjugated
• Cellular Localization: Cytoplasm, Mitochondrion, Nucleus, Cell Junction, Synapse, Presynaptic Cell Membrane
• Certificate of Analysis: 0.1 ug/ml was sufficient for detection of CamKII in 20 ug rat brain tissue extract by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.
• Preparation and Storage: Store at-20 degree C.

Immunocytochemistry (ICC)

(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9 . Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at-20 degree C. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear Staining.)

Western Blot (WB)

(Western Blot analysis of Rat brain membrane lysate showing detection of CaMKII protein using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9 . Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:1000.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9 . Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Hair follicles, epidermis.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9 . Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Magnification: 40x.)

Immunocytochemistry (ICC)/Immunofluorescence (IF)

(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9 . Tissue: dissociated hippocampal neurons. Species: Mouse. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:1000 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Magnification: 10X. Courtesy of: Mary Kennedy, Caltech.)

Related Product Information for anti-CaMKII antibody

CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).

Product Categories/Family for anti-CaMKII antibody

Cell Signaling; Post-translational Modifications; Phosphorylation

References

1. Hughes K. et al. (2001) J. Biol. Chem. 276: 36008-36013. 2. Barria A. et al. (1997) Science 276: 2042-2045. 3. Bennet M.K. and Kennedy M.B. (1987) Proc. Natl. Acad. Sci. U.S.A. 84: 1794-1798. 4. Broke L., Srinivasan M. and Schulman H. (1995) J. Neurosci. 15: 6797-6808. 5. Nghiem P., Saati S. M., Martens C. L., Gardner P. and Schulman H. (1993) J. Biol. Chem. 268: 5471-5479. 6. Edman C.F. and Schulman H. (1994) Biochem. Biophys. Acta 1221: 90-102. 7. Tombes R.M. and Krystal G.W., (1997) Biochem. Biophys. Acta 13555: 281-292. 8. Means A.R. (2000) Mol. Endocrinol. 14: 4-12. 9. Makhinson M. et al. (1999) J. Neurosci. 19: 2500-2510. 10. Strack S. and Colbran R.J. (1998) J. Biol. Chem. 273: 20689-20692. 11. Leonard S.A., Lim I.A., Hemsworth D.E., Horne M.C. and Hell J.W. (1999) Proc. Natl. Acad. Sci. U.S.A. 96: 3239-3244. 12. Shen K. and Meyer Y. (1999) Science 284: 162-167.

NCBI and Uniprot Product Information

• NCBI GI #: 124056467
• NCBI GeneID: 12322
• UniProt Accession #: P11798
• NCBI Official Full Name: Calcium/calmodulin-dependent protein kinase type II subunit alpha
• NCBI Official Synonym Full Names: calcium/calmodulin-dependent protein kinase II alpha
• NCBI Official Symbol: Camk2a
• NCBI Official Synonym Symbols: CaMKII; R74975; mKIAA0968
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase type II subunit alpha
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase type II subunit alpha
• Protein Family: Calcium/calmodulin-dependent protein kinase
• UniProt Gene Name: Camk2a
• UniProt Synonym Gene Names: CaM kinase II subunit alpha; CaMK-II subunit alpha
• UniProt Entry Name: KCC2A_MOUSE

Uniprot Description

CAMK2A: a protein kinase of the CAMK2 family. A prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses that may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. The holoenzyme is composed of four different chains: alpha, beta, gamma, and delta. The different chains assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. May interact with BAALC, MPDZ, SYN1 and synGAP. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.11.17; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; CAMK group; CAMK2 family

Cellular Component: dendrite cytoplasm; mitochondrion; cell soma; membrane; axon; cytoplasm; dendrite; postsynaptic density; plasma membrane; synapse; cell junction; nucleus

Molecular Function: glutamate receptor binding; protein homodimerization activity; calmodulin-dependent protein kinase activity; nucleotide binding; GTPase activating protein binding; protein kinase activity; calmodulin binding; transferase activity; protein serine/threonine kinase activity; protein binding; transferase activity, transferring phosphorus-containing groups; kinase activity; ATP binding

Biological Process: peptidyl-serine phosphorylation; ionotropic glutamate receptor signaling pathway; protein amino acid autophosphorylation; calcium ion transport; regulation of neurotransmitter secretion; positive regulation of calcium ion transport; protein amino acid phosphorylation; phosphorylation; G1/S transition of mitotic cell cycle; activation of NF-kappaB transcription factor; regulation of neuronal synaptic plasticity