Mouse anti-Rat, Human CaMKII Monoclonal Antibody | anti-Camk2a antibody

CaMKII Antibody: PerCP

Cat. Num. AAA804903

• Gene Names: Camk2a; PKCCD; PK2CDD
• Reactivity: Rat, Human
• Applications: ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
• Synonyms: CaMKII; Monoclonal Antibody; CaMKII Antibody: PerCP; CamK2; CamK2A; CamK2B; CamK2D; CamK2G; CAMKA; anti-Camk2a antibody

• Host: Mouse
• Reactivity: Rat, Human
• Clonality: Monoclonal
• Isotype: IgG1 Kappa
• Clone Number: 22B1
• Form/Format: Protein G Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 478

• Applicable Applications for anti-Camk2a antibody: ELISA (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: 1ug/ml was sufficient for detection of 0.2ug CAMKII by colorimetric immunoblot
• Conjugate: PerCP
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Preparation and Storage: -20 degree C

Immunocytochemistry/Immunofluorescence (ICC/IF)

(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1. Tissue: dissociated hippocampal neurons. Species: Rat. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:1000 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Magnification: 10X. Courtesy of: Mary Kennedy, Caltech.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Muscle, hair follicle, epidermis. Backskin obtained from transgenic mice.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:5000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Magnification: 40x.)

Western Blot (WB)

(Western Blot analysis of Mouse Ventricle lysates showing detection of CaMKII protein using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:1000. Analysis of CaMKII and NFAT phosphorylation in ventricles of 14 day old mice over-expressing CaMK.)

Related Product Information for anti-Camk2a antibody

Background Info: Detects phosphorylated CaMKII from rat tissues. This antibody is specific for alpha and beta subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in beta).

Scientific Background: CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).

Product Categories/Family for anti-Camk2a antibody

Cell Signaling

References

1. Hughes K. et al. (2001) J. Biol. Chem. 276: 36008-36013. 2. Barria A. et al. (1997) Science 276: 2042-2045. 3. Bennet M.K. and Kennedy M.B. (1987) Proc. Natl. Acad. Sci. U.S.A. 84: 1794-1798. 4. Broke L., Srinivasan M. and Schulman H. (1995) J. Neurosci. 15: 6797-6808. 5. Nghiem P., Saati S. M., Martens C. L., Gardner P. and Schulman H. (1993) J. Biol. Chem. 268: 5471-5479. 6. Edman C.F. and Schulman H. (1994) Biochem. Biophys. Acta 1221: 90-102. 7. Tombes R.M. and Krystal G.W., (1997) Biochem. Biophys. Acta 13555: 281-292. 8. Means A.R. (2000) Mol. Endocrinol. 14: 4-12. 9. Makhinson M. et al. (1999) J. Neurosci. 19: 2500-2510. 10. Strack S. and Colbran R.J. (1998) J. Biol. Chem. 273: 20689-20692. 11. Leonard S.A., Lim I.A., Hemsworth D.E., Horne M.C. and Hell J.W. (1999) Proc. Natl. Acad. Sci. U.S.A. 96: 3239-3244. 12. Shen K. and Meyer Y. (1999) Science 284: 162-167.
1. Feng, T., Szabo, E., Dziak, E. and Opas, M. (2010). Cytoskeletal disassembly and cell rounding promotes adipogenesis from ES cells. Stem Cell Rev. 6 (1), 74-85. doi: 10.1007/s12015-010-9115-8.

NCBI and Uniprot Product Information

• NCBI GI #: 6978593
• NCBI GeneID: 25400
• NCBI Accession #: NP_037052.1
• NCBI GenBank Nucleotide #: NM_012920.1
• UniProt Accession #: P11275
• Molecular Weight: 54,115 Da
• NCBI Official Full Name: calcium/calmodulin-dependent protein kinase type II subunit alpha
• NCBI Official Synonym Full Names: calcium/calmodulin-dependent protein kinase II alpha
• NCBI Official Symbol: Camk2a
• NCBI Official Synonym Symbols: PKCCD; PK2CDD
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase type II subunit alpha; alpha CaM kinase II; caMK-II subunit alpha; caM-kinase II alpha chain; caM kinase II subunit alpha; Ca2+/calmodulin-dependent protein kinase II alpha; calcium/calmodulin-dependent protein kinase II alpha subunit; calcium/calmodulin-dependent protein kinase type II alpha chain; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase type II subunit alpha
• Protein Family: Calcium/calmodulin-dependent protein kinase
• UniProt Gene Name: Camk2a
• UniProt Synonym Gene Names: CaM kinase II subunit alpha; CaMK-II subunit alpha
• UniProt Entry Name: KCC2A_RAT

Uniprot Description

CAMK2A: a protein kinase of the CAMK2 family. A prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses that may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. The holoenzyme is composed of four different chains: alpha, beta, gamma, and delta. The different chains assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. May interact with BAALC, MPDZ, SYN1 and synGAP. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.11.17; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; CAMK group; CAMK2 family

Cellular Component: dendrite cytoplasm; presynaptic membrane; cell soma; mitochondrion; axon; postsynaptic density; dendrite; nucleus; cell junction

Molecular Function: calmodulin binding; protein serine/threonine kinase activity; protein binding; glutamate receptor binding; protein homodimerization activity; calmodulin-dependent protein kinase activity; GTPase activating protein binding; kinase activity; ATP binding

Biological Process: peptidyl-serine phosphorylation; ionotropic glutamate receptor signaling pathway; calcium ion transport; protein amino acid autophosphorylation; regulation of neurotransmitter secretion; positive regulation of calcium ion transport; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; G1/S transition of mitotic cell cycle; regulation of neuronal synaptic plasticity