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Western Blot (WB) (Western blot analysis of extracts from Jurkat, A549, MCF7, C6 and Hela cell lysates using CaMKII mouse mAb (1:1000 diluted).Predicted band size:63KDa.Observed band size:63KDa.)

Mouse anti-Human, Rat CaMKII Monoclonal Antibody | anti-CaMKII antibody

Anti-CaMKII Mouse mAb

Gene Names
CAMK2G; CAMK; CAMKG; CAMK-II
Reactivity
Human, Rat
Applications
Western Blot
Synonyms
CaMKII; Monoclonal Antibody; Anti-CaMKII Mouse mAb; x Calcium/calmodulin dependent protein kinase II alpha; Calcium/calmodulin dependent protein kinase II beta; Calcium/calmodulin dependent protein kinase II delta; Calcium/calmodulin dependent protein kinase II gamma; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II alpha; CaM kinase II; CaM kinase II beta; CaM kinase II delta; CaM kinase II gamma; CaM kinase II subunit alpha; CaMK-II subunit alpha; CAMK2; Camk2a; CAMK2B; CAMK2D; CAMK2G; CAMKA; KCC2A_HUMAN; anti-CaMKII antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3D1-G8-D5
Form/Format
Purified antibody in PBS with 0.05% sodium azide.
Sequence Length
495
Applicable Applications for anti-CaMKII antibody
Western Blot (WB)
Immunogen
Purified recombinant fragment of human CAMK2G (AA: 322-481) expressed in E Coli.
Sequence Similarities
Contains 1 protein kinase domain.
Subunit Structure
CAMK2 is composed of 4 different chains: alpha (CAMK2A), beta (CAMK2B), gamma (CAMK2G), and delta (CAMK2D). The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other.
Subcellular Location
Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side

Western Blot (WB)

(Western blot analysis of extracts from Jurkat, A549, MCF7, C6 and Hela cell lysates using CaMKII mouse mAb (1:1000 diluted).Predicted band size:63KDa.Observed band size:63KDa.)

Western Blot (WB) (Western blot analysis of extracts from Jurkat, A549, MCF7, C6 and Hela cell lysates using CaMKII mouse mAb (1:1000 diluted).Predicted band size:63KDa.Observed band size:63KDa.)

Western Blot (WB)

(Western blot analysis using CAMK2G mAb against HEK293 (1) and CAMK2G (AA)

Western Blot (WB) (Western blot analysis using CAMK2G mAb against HEK293 (1) and CAMK2G (AA)

Western Blot (WB)

(Western blot analysis using CAMK2G mouse mAb against PC-12 (1), Jurkat (2), T47D (3), HepG2 (4) cell lysate.)

Western Blot (WB) (Western blot analysis using CAMK2G mouse mAb against PC-12 (1), Jurkat (2), T47D (3), HepG2 (4) cell lysate.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Jurkat cells using CAMK2G mouse mAb (green) and negative control (red).)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of Jurkat cells using CAMK2G mouse mAb (green) and negative control (red).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using CAMK2G mouse mAb with DAB staining.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using CAMK2G mouse mAb with DAB staining.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CAMK2G mouse mAb with DAB staining.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CAMK2G mouse mAb with DAB staining.)
Related Product Information for anti-CaMKII antibody
Entrez Summary: The product of this gene is one of the four subunits of an enzyme which belongs to the serine/threonine protein kinase family, and to the Ca (2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a gamma chain. Many alternatively spliced transcripts encoding different isoforms have been described but the full-length nature of all the variants has not been determined.[provided by RefSeq, Mar 2011]
UniPort Summary Function: Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca (2+)/calmodulin-binding and autophosphorylation, and is involved in sarcoplsamic reticulum Ca (2+) transport in skeletal muscle and may function in dendritic spine and synapse formation and neuronal plasticity. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca (2+) transport and in fast-twitch muscle participates in the control of Ca (2+) release from the SR through phosphorylation of the ryanodine receptor-coupling factor triadin. In neurons, may participate in the promotion of dendritic spine and synapse formation and maintenance of synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
818
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
63kDa
NCBI Official Full Name
calcium/calmodulin-dependent protein kinase type II subunit gamma isoform 4
NCBI Official Synonym Full Names
calcium/calmodulin dependent protein kinase II gamma
NCBI Official Symbol
CAMK2G
NCBI Official Synonym Symbols
CAMK; CAMKG; CAMK-II
NCBI Protein Information
calcium/calmodulin-dependent protein kinase type II subunit gamma
UniProt Protein Name
Calcium/calmodulin-dependent protein kinase type II subunit gamma
UniProt Gene Name
CAMK2G
UniProt Synonym Gene Names
CAMK; CAMK-II; CAMKG; CaM kinase II subunit gamma; CaMK-II subunit gamma

NCBI Description

The product of this gene is one of the four subunits of an enzyme which belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a gamma chain. Many alternatively spliced transcripts encoding different isoforms have been described but the full-length nature of all the variants has not been determined.[provided by RefSeq, Mar 2011]

Uniprot Description

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca2+/calmodulin-binding and autophosphorylation, and is involved in sarcoplsamic reticulum Ca2+ transport in skeletal muscle and may function in dendritic spine and synapse formation and neuronal plasticity. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca2+ transport and in fast-twitch muscle participates in the control of Ca2+ release from the SR through phosphorylation of the ryanodine receptor-coupling factor triadin. In neurons, may participate in the promotion of dendritic spine and synapse formation and maintenance of synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning.

Research Articles on CaMKII

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Product Notes

The CaMKII camk2g (Catalog #AAA476530) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CaMKII Mouse mAb reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CaMKII can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the CaMKII camk2g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CaMKII, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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